Xiao-Ai-Ping, a TCM Injection, Enhances the Antigrowth Effects of Cisplatin on Lewis Lung Cancer Cells through Promoting the Infiltration and Function of CD8(+) T Lymphocytes.
Xiao-Ai-Ping, a TCM Injection, Enhances the Antigrowth Effects of Cisplatin on Lewis Lung Cancer Cells through Promoting the Infiltration and Function of CD8(+) T Lymphocytes.
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DOI：
10.1155/2013/879512
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Li W;Yang Y;Ouyang Z;Zhang Q;Wang L;Tao F;Shu Y;Gu Y;Xu Q;Sun Y
目标。探讨中药消癌平注射液作为肿瘤治疗的辅助药物如何增强顺铂对Lewis肺癌(LLC)细胞的抗肿瘤作用。方法。 LLC荷瘤小鼠每天分别腹腔注射不同剂量的顺铂、消癌平或顺铂加消癌平。每三天测量一次体重和肿瘤体积。结果。消癌平与顺铂联用对 LLC 异种移植物产生比单独药物治疗显着更好的抗生长和促凋亡作用。此外，我们发现小爱平触发了CD8+ T细胞（一组细胞毒性T细胞）对LLC异种移植物的浸润。此外，消癌平与顺铂联合治疗后，CD8+ T细胞中干扰素-γ（ifn-γ）、穿孔素-1（prf-1）和颗粒酶B（gzmb）的mRNA水平显着增加。体外研究表明，小爱平以浓度依赖性方式显着上调CD8+ T细胞中ifn-γ、prf-1和gzmb的mRNA水平，表明小爱平增强了CD8+ T细胞的功能。结论。消癌平促进CD8+T细胞的浸润和功能，从而增强顺铂对LLC异种移植物的抗生长作用，这为消癌平与顺铂在我国临床的联合应用提供了新的证据。
Objectives. To investigate how Xiao-Ai-Ping injection, a traditional Chinese medicine and an ancillary drug in tumor treatment, enhances the antitumor effects of cisplatin on Lewis lung cancer (LLC) cells. Methods. LLC-bearing mice were daily intraperitoneally injected with various doses of cisplatin, Xiao-Ai-Ping, or cisplatin plus Xiao-Ai-Ping, respectively. Body weight and tumor volumes were measured every three days. Results. Combination of Xiao-Ai-Ping and cisplatin yielded significantly better antigrowth and proapoptotic effects on LLC xenografts than sole drug treatment did. In addition, we found that Xiao-Ai-Ping triggered the infiltration of CD8+ T cells, a group of cytotoxic T cells, to LLC xenografts. Furthermore, the mRNA levels of interferon-γ (ifn- γ), perforin-1 (prf-1), and granzyme B (gzmb) in CD8+ T cells were significantly increased after combination treatment of Xiao-Ai-Ping and cisplatin. In vitro studies showed that Xiao-Ai-Ping markedly upregulated the mRNA levels of ifn- γ, prf-1, and gzmb in CD8+ T cells in a concentration-dependent manner, suggesting that Xiao-Ai-Ping augments the function of CD8+ T cells. Conclusions. Xiao-Ai-Ping promotes the infiltration and function of CD8+ T cells and thus enhances the antigrowth effects of cisplatin on LLC xenografts, which provides new evidence for the combination of Xiao-Ai-Ping and cisplatin in clinic in China.
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影响因子：
11.2
作者：
Kang TH;Mao CP;Lee SY;Chen A;Lee JH;Kim TW;Alvarez RD;Roden RB;Pardoll D;Hung CF;Wu TC
通讯作者：
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2.8
作者：
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影响因子：
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作者：
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通讯作者：
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