Endothelial cell layer subjected to impinging flow mimicking the apex of an arterial bifurcation.
Endothelial cell layer subjected to impinging flow mimicking the apex of an arterial bifurcation.
复制标题
DOI：
10.1007/s10439-008-9540-x
复制
发表时间：
2008-10
影响因子：
3.8
通讯作者：
Kolega, John
中科院分区：
文献类型：
--
作者：
Szymanski, Michael P.;Metaxa, Eleni;Meng, Hui;Kolega, John
血管内皮细胞对发生在动脉分叉处的撞击血流动力学的反应知之甚少，而动脉分叉处通常会形成颅内动脉瘤。这种血流动力学环境的特点是高壁面剪应力(WSS&>40dynes/cm2)和高壁面剪应力梯度(WSSG>；300dynes/cm2)。在这项研究中，汇合的牛主动脉内皮细胞暴露在模拟分叉的T形室中的撞击流中。24−72 h后，停滞点周围的细胞保持多边形形态，但细胞密度降低，而邻近下游区域的细胞被拉长，平行于流动排列，密度较高。这种行为不能通过抑制增殖来阻止，这表明细胞在撞击流的作用下从停滞点向下游迁移。此外，尽管细胞密度最高的区域随着时间的推移向下游移动并远离撞击点，但它从未超过WSS最大值。最大WSS上游和最大WSSG下游的细胞聚集表明WSSG阳性是导致观察到的迁移的原因。这些结果表明，在分叉处，内皮细胞对促进动脉瘤的流动环境有独特的反应。
Little is known about endothelial responses to the impinging flow hemodynamics that occur at arterial bifurcation apices, where intracranial aneurysms usually form. Such hemodynamic environments are characterized by high wall shear stress (WSS >40 dynes/cm2) and high wall shear stress gradients (WSSG >300 dynes/cm3). In this study, confluent bovine aortic endothelial cells were exposed to impinging flow in a T-shaped chamber designed to mimic a bifurcation. After 24−72 h under flow, cells around the stagnation point maintained polygonal shapes but cell density was reduced, whereas cells in adjacent downstream regions exposed to very high WSS and WSSG were elongated, aligned parallel to flow, and at higher density. Such behavior was not blocked by inhibiting proliferation, indicating that cells migrated downstream from the stagnation point in response to impinging flow. Furthermore, although the area of highest cell density moved downstream and away from the impingement point over time, it never moved beyond the WSS maximum. The accumulation of cells upstream of maximal WSS and downstream of maximal WSSG suggests that positive WSSG is responsible for the observed migration. These results demonstrate a unique endothelial response to aneurysm-promoting flow environments at bifurcation apices.
登录
查看更多内容
影响因子：
8.3
作者：
Shojima, M;Oshima, M;Kirino, T
通讯作者：
Kirino, T
影响因子：
2.4
作者：
Chiu, JJ;Chen, CN;Usami, S
通讯作者：
Usami, S
影响因子：
8.3
作者：
Chien, S;Li, S;Shyy, JYJ
通讯作者：
Shyy, JYJ
影响因子：
4.1
作者：
Hoi, YM;Meng, H;Hopkins, LN
通讯作者：
Hopkins, LN
影响因子：
--
作者：
STEHBENS, WE
通讯作者：
STEHBENS, WE