RNA polymerase mutants defective in the initiation of transcription-coupled DNA repair.
RNA polymerase mutants defective in the initiation of transcription-coupled DNA repair.
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DOI：
10.1093/nar/gki225
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发表时间：
2005
影响因子：
14.9
通讯作者：
Savery NJ
中科院分区：
文献类型：
--
作者：
Smith AJ;Savery NJ
细菌MFD蛋白是一种转录修复偶联因子，在转录偶联DNA修复过程中发挥两个关键功能。第一种是将因DNA损伤而停滞不前的RNA聚合酶(RNAP)复合体从损伤部位移除，第二种是介导DNA修复蛋白的募集。MFD还取代了因蛋白质障碍而停滞不前的转录复合体，并催化了已“回溯”的转录复合体的重新激活。我们已经在大肠杆菌RNAP的β亚单位中发现了氨基酸替换，这种替换破坏了MFD和RNAP之间的直接相互作用。这些替换防止MFD在体内和体外将停滞的RNAP从DNA中置换出来。他们在RNAP1结构域上定义了一个高度保守的表面暴露的斑块，这是β在转录偶联修复的初始步骤、增强路障抑制和重新激活回溯的转录复合体所必需的。
The bacterial Mfd protein is a transcription-repair coupling factor that performs two key functions during transcription-coupled DNA repair. The first is to remove RNA polymerase (RNAP) complexes that have been stalled by a DNA lesion from the site of damage, and the second is to mediate the recruitment of DNA repair proteins. Mfd also displaces transcription complexes that have been stalled by protein roadblocks, and catalyses the reactivation of transcription complexes that have become ‘backtracked’. We have identified amino acid substitutions in the β subunit of Escherichia coli RNAP that disrupt a direct interaction between Mfd and RNAP. These substitutions prevent Mfd displacing stalled RNAP from DNA in vivo and in vitro. They define a highly conserved surface-exposed patch on the β1 domain of RNAP that is required by Mfd for the initial step of transcription-coupled repair, the enhancement of roadblock repression and the reactivation of backtracked transcription complexes.
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