Salvianolic acid B protects hepatocytes from H(2)O(2) injury by stabilizing the lysosomal membrane.
Salvianolic acid B protects hepatocytes from H(2)O(2) injury by stabilizing the lysosomal membrane.
复制标题
丹酚酸 B 通过稳定溶酶体膜保护肝细胞免受 H2O2 损伤
DOI：
10.3748/wjg.v23.i29.5333
复制
发表时间：
2017-08-07
影响因子：
4.3
通讯作者：
Wang XL
中科院分区：
文献类型：
--
作者：
Yan XF;Zhao P;Ma DY;Jiang YL;Luo JJ;Liu L;Wang XL
目的研究丹酚酸B(SalB)对过氧化氢(H2O2)/四氯化碳(CCl4)诱导的肝细胞溶酶体膜通透性的影响。方法细胞计数Kit-8法检测细胞存活率。流式细胞仪检测细胞凋亡率和死亡率。BrdU掺入法检测细胞增殖情况。测定血清丙氨酸氨基转移酶活性和肝脏丙二醛(MDA)含量。苏木精-伊红染色观察肝组织病理变化。溶酶体膜通透性检测采用LysoTracker Green标记探针和丫啶橙染色。用免疫印迹法检测蛋白质羰基含量(PCC)、组织蛋白(Cat)B/D和溶酶体相关膜蛋白1(LAMP1)水平。采用化学发光法进行胞浆CatB活性分析。用实时定量聚合酶链式反应检测LAMP1基因的表达水平。结果H_2O_2可诱导细胞损伤/死亡。丹参B通过增加LAMP1蛋白水平，拮抗CatB/D向胞浆的渗漏，减轻过氧化氢诱导的细胞凋亡和死亡，恢复对细胞增殖的抑制，减少PCC的生成，稳定溶酶体膜。CCl4还引发了肝细胞死亡。此外，SAL B通过增加LAMP1的表达和减少溶酶体酶向胞浆的移位，有效地挽救了肝细胞。结论丹参皂苷B通过稳定溶酶体膜对小鼠胚胎肝细胞的损伤/死亡具有保护作用。
AIM To investigate the capability of salvianolic acid B (Sal B) to protect hepatocytes from hydrogen peroxide (H2O2)/carbon tetrachloride (CCl4)-induced lysosomal membrane permeabilization. METHODS Cell Counting Kit-8 assay was used to measure cell viability. Apoptosis and death were assayed through flow cytometry. BrdU incorporation was used to detect cell proliferation. Serum alanine aminotransferase activity and liver malondialdehyde (MDA) content were measured. Liver histopathological changes were evaluated using hematoxylin-eosin staining. Lysosomal membrane permeability was detected with LysoTracker Green-labeled probes and acridine orange staining. The levels of protein carbonyl content (PCC), cathepsins (Cat)B/D, and lysosome-associated membrane protein 1 (LAMP1) were evaluated through western blotting. Cytosol CatB activity analysis was performed with chemiluminescence detection. The mRNA level of LAMP1 was evaluated through quantitative real-time polymerase chain reaction. RESULTS Results indicated that H2O2 induced cell injury/death. Sal B attenuated H2O2-induced cell apoptosis and death, restored the inhibition of proliferation, decreased the amount of PCC, and stabilized the lysosome membrane by increasing the LAMP1 protein level and antagonizing CatB/D leakage into the cytosol. CCl4 also triggered hepatocyte death. Furthermore, Sal B effectively rescued hepatocytes by increasing LAMP1 expression and by reducing lysosomal enzyme translocation to the cytosol. CONCLUSION Sal B protected mouse embryonic hepatocytes from H2O2/CCl4-induced injury/death by stabilizing the lysosomal membrane.
登录
查看更多内容
DOI：
10.1186/1756-9966-31-41
发表时间：
2012-05-02
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Hornick JR;Vangveravong S;Spitzer D;Abate C;Berardi F;Goedegebuure P;Mach RH;Hawkins WG
通讯作者：
Hawkins WG
DOI：
10.4137/jcd.s39824
发表时间：
2016
期刊：
Journal of cell death
影响因子：
--
作者：
Cao L;Quan XB;Zeng WJ;Yang XO;Wang MJ
通讯作者：
Wang MJ
生态系统服务的空间多尺度关系：使用地统计学方法的案例研究。
影响因子：
4.6
作者：
Liu Y;Bi J;Lv J;Ma Z;Wang C
通讯作者：
Wang C
影响因子：
6.2
作者：
Lee, Sook-Jeong;Park, Mi-Ha;Koh, Jae-Young
通讯作者：
Koh, Jae-Young
影响因子：
5.6
作者：
Li W;Qu XN;Han Y;Zheng SW;Wang J;Wang YP
通讯作者：
Wang YP