Ameliorative effects of 5-hydroxymethyl-2-furfural (5-HMF) from Schisandra chinensis on alcoholic liver oxidative injury in mice.
Ameliorative effects of 5-hydroxymethyl-2-furfural (5-HMF) from Schisandra chinensis on alcoholic liver oxidative injury in mice.
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DOI：
10.3390/ijms16022446
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发表时间：
2015-01-22
影响因子：
5.6
通讯作者：
Wang YP
中科院分区：
文献类型：
--
作者：
Li W;Qu XN;Han Y;Zheng SW;Wang J;Wang YP
研究5-羟甲基糠醛（5-HMF）对急性酒精性肝氧化损伤的保护作用。从北五味子果实中分离得到美拉德反应产物5-羟甲基糠醛，用于动物实验。实验ICR小鼠通过灌胃喂食用不同剂量的5-HMF（7.5、15和30 mg/kg）预处理7天。检测血清和肝组织的生化指标和抗氧化酶。结果表明：与酒精组相比，5-HMF处理组血清ALT、AST、TC、TG、L-DLC活性及肝组织MDA含量均显著降低（p < 0.05）。相反，酶抗氧化剂CAT（过氧化氢酶）、GSH-Px（谷胱甘肽过氧化物酶）和GSH SOD（超氧化物歧化酶）在5-HMF处理的肝组织中显著升高（p < 0.05）。此外，促炎反应标志物肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1β）的肝脏水平被显著抑制（p < 0.05）。组织学检查显示5-HMF（30 mg/kg）预处理可显著防止酒精诱导的肝细胞凋亡和脂肪变性。这表明，5-HMF对酒精诱导的肝氧化损伤的保护作用可能是由于其强大的抗氧化特性。
The aim of this paper is to evaluate the protective effect of 5-hydroxymethyl-2-furfural (5-HMF) on acute alcohol-induced liver oxidative injury in mice. 5-HMF, a maillard reaction product, was isolated from the fruits of Schisandra chinensis for animal experiments. Experimental ICR mice were pretreated with different doses of 5-HMF (7.5, 15, and 30 mg/kg) for seven days by gavage feeding. Biochemical markers and enzymatic antioxidants from serum and liver tissue were examined. Our results showed that the activities of ALT (alanine aminotransferase), AST (aspartate transaminase), TC (total cholesterol), TG (triglyceride), L-DLC (low density lipoprotein) in serum and the levels of MDA (malondialdehyde) in liver tissue, decreased significantly (p < 0.05) in the 5-HMF-treated group compared with the alcohol group. On the contrary, enzymatic antioxidants CAT (catalase), GSH-Px (glutathione peroxidase), and GSH SOD (superoxide dismutase) were markedly elevated in liver tissue treated with 5-HMF (p < 0.05). Furthermore, the hepatic levels of pro-inflammatory response marker tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) were significantly suppressed (p < 0.05). Histopathological examination revealed that 5-HMF (30 mg/kg) pretreatment noticeably prevented alcohol-induced hepatocyte apoptosis and fatty degeneration. It is suggested that the hepatoprotective effects exhibited by 5-HMF on alcohol-induced liver oxidative injury may be due to its potent antioxidant properties.
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影响因子：
4.6
作者：
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