A complex network of additive and epistatic quantitative trait loci underlies natural variation of Arabidopsis thaliana quantitative disease resistance to Ralstonia solanacearum under heat stress.
A complex network of additive and epistatic quantitative trait loci underlies natural variation of Arabidopsis thaliana quantitative disease resistance to Ralstonia solanacearum under heat stress.
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DOI：
10.1111/mpp.12964
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发表时间：
2020-11
影响因子：
4.9
通讯作者：
Roux F
中科院分区：
文献类型：
--
作者：
Aoun N;Desaint H;Boyrie L;Bonhomme M;Deslandes L;Berthomé R;Roux F
植物的免疫力经常受到热胁迫的负面影响。然而，潜在的分子机制仍然很差的特点。基于全基因组关联作图方法，本研究旨在确定拟南芥在热胁迫下对毁灭性病原菌青枯雷尔氏菌（Ralstonia solanacearum）的强大抗性机制的遗传基础。在27 ° C和30 °C下针对青枯菌GMI1000菌株对局部作图群体进行表型分析。为了获得对数量抗病性（QDR）自然变异的遗传结构的精确描述，我们应用了全基因组局部评分分析。除了在这两种温度下在当地人群中发现的广泛的遗传变异外，我们还观察到了数量性状基因座沿着感染阶段的有趣动态。此外，在30 °C下可以检测到相互作用位点的复杂遗传网络。作为研究潜在分子机制的第一步，通过反向遗传方法验证了非典型减数分裂细胞周期蛋白SOLO DANCERS基因在30 °C下参与青枯菌的QDR。在气候变化的背景下，复杂的遗传结构下的QDR热应力在当地的映射人群揭示了候选基因具有不同的分子功能。拟南芥在热胁迫下对青枯雷尔氏菌响应的自然变异的全基因组关联研究揭示了由加性和上位性数量性状基因座的复杂遗传网络所支撑的弹性数量抗病性。
Plant immunity is often negatively impacted by heat stress. However, the underlying molecular mechanisms remain poorly characterized. Based on a genome‐wide association mapping approach, this study aims to identify in Arabidopsis thaliana the genetic bases of robust resistance mechanisms to the devastating pathogen Ralstonia solanacearum under heat stress. A local mapping population was phenotyped against the R. solanacearum GMI1000 strain at 27 and 30 °C. To obtain a precise description of the genetic architecture underlying natural variation of quantitative disease resistance (QDR), we applied a genome‐wide local score analysis. Alongside an extensive genetic variation found in this local population at both temperatures, we observed a playful dynamics of quantitative trait loci along the infection stages. In addition, a complex genetic network of interacting loci could be detected at 30 °C. As a first step to investigate the underlying molecular mechanisms, the atypical meiotic cyclin SOLO DANCERS gene was validated by a reverse genetic approach as involved in QDR to R. solanacearum at 30 °C. In the context of climate change, the complex genetic architecture underlying QDR under heat stress in a local mapping population revealed candidate genes with diverse molecular functions. A genome‐wide association study of the natural variation of Arabidopsis thaliana response to Ralstonia solanacearum under heat stress revealed resilient quantitative disease resistance underlain by a complex genetic network of additive and epistatic quantitative trait loci.
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影响因子：
16.6
作者：
Cheng, Cheng;Gao, Xiquan;Feng, Baomin;Sheen, Jen;Shan, Libo;He, Ping
通讯作者：
He, Ping
影响因子：
5.6
作者：
Bita CE;Gerats T
通讯作者：
Gerats T
拟南芥种群内 R 基因变异的独特模式
影响因子：
3.1
作者：
Ding, Jing;Zhang, Weili;Tian, Dacheng
通讯作者：
Tian, Dacheng
影响因子：
30.8
作者：
Carlborg, Ö;Jacobsson, L;Andersson, L
通讯作者：
Andersson, L
影响因子：
4.9
作者：
Debieu, Marilyne;Huard-Chauveau, Carine;Roby, Dominique
通讯作者：
Roby, Dominique