Photoinitiated polymerization of PEG-diacrylate with lithium phenyl-2,4,6-trimethylbenzoylphosphinate: polymerization rate and cytocompatibility.
Photoinitiated polymerization of PEG-diacrylate with lithium phenyl-2,4,6-trimethylbenzoylphosphinate: polymerization rate and cytocompatibility.
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DOI：
10.1016/j.biomaterials.2009.08.055
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发表时间：
2009-12
期刊：
影响因子：
14
通讯作者：
Anseth, Kristi S.
中科院分区：
文献类型：
--
作者：
Fairbanks, Benjamin D.;Schwartz, Michael P.;Bowman, Christopher N.;Anseth, Kristi S.
由于温和的反应条件和对材料形成的时间和空间控制，光聚合已成为在三维、水合、仿生材料中包封活细胞的有价值的技术。对于这样的应用，2-羟基-1-[4-（2-羟基乙氧基）苯基]-2-甲基-1-丙酮（I2959）是最常用的光引发剂（由于其中等的水溶性），然而该引发剂具有与通常被认为对活细胞有益的光的波长匹配不良的吸收光谱，限制了其在活细胞存在下引发聚合的速率。相比之下，酰基氧化膦光引发剂通常在适合于细胞包封的波长下表现出吸收光谱，然而这类市售引发剂具有低水溶性。在此，评价水溶性酰基次膦酸锂盐将二丙烯酸酯化聚（乙二醇）（PEGDA）单体快速包封到水凝胶中，同时在细胞的直接包封期间保持高活力的能力。通过流变测量，当暴露于365 nm光时，具有次膦酸盐引发剂的PEGDA溶液达到凝胶化的时间是使用类似浓度的I2959的时间的十分之一。此外，在低引发剂浓度和光强度下实现了在405 nm可见光暴露下用次膦酸盐引发剂的聚合，这在用I2959引发的聚合中由于其吸光度曲线而被排除。当在包封后24小时检查时，包封在与次膦酸盐引发剂聚合的水凝胶中的人新生儿成纤维细胞的存活率超过95%，证明了该引发系统的细胞相容性。
Due to mild reaction conditions and temporal and spatial control over material formation, photopolymerization has become a valuable technique for the encapsulation of living cells in three dimensional, hydrated, biomimetic materials. For such applications,2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone (I2959) is the most commonly used photoinitiator (by virtue of its moderate water solubility), yet this initiator has an absorption spectrum that is poorly matched with wavelengths of light generally regarded as benign to living cells, limiting the rate at which it may initiate polymerization in their presence. In contrast, acylphosphine oxide photoinitiators, generally exhibit absorption spectra at wavelengths suitable for cell encapsulation, yet commercially available initiators of this class have low water solubility. Here, a water soluble lithium acylphosphinate salt is evaluated for its ability to polymerize diacrylated poly(ethylene glycol) (PEGDA) monomers rapidly into hydrogels, while maintaining high viability during direct encapsulation of cells. Through rheometric measurements, the time to reach gelation of a PEGDA solution with the phosphinate initiator is one tenth the time for that using I2959 at similar concentrations, when exposed to 365 nm light. Further, polymerization with the phosphinate initiator at 405 nm visible light exposure is achieved with low initiator concentrations and light intensities, precluded in polymerizations initiated with I2959 by its absorbance profile. When examined 24 hours after encapsulation, survival rates of human neonatal fibroblasts encapsulated in hydrogels polymerized with the phosphinate initiator exceed 95%, demonstrating the cytocompatibility of this initiating system.
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影响因子：
--
作者：
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作者：
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