Effect of Ethanol Vapor Treatment on the Growth of Alternaria alternata and Botrytis cinerea and Defense-Related Enzymes of Fungi-Inoculated Blueberry During Storage.
Effect of Ethanol Vapor Treatment on the Growth of Alternaria alternata and Botrytis cinerea and Defense-Related Enzymes of Fungi-Inoculated Blueberry During Storage.
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DOI：
10.3389/fmicb.2021.618252
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发表时间：
2021
影响因子：
5.2
通讯作者：
Saren G
中科院分区：
文献类型：
--
作者：
Ji Y;Hu W;Liao J;Xiu Z;Jiang A;Yang X;Guan Y;Feng K;Saren G
本试验研究了乙醇蒸汽对蓝莓采后真菌侵染后在0±0.5°C条件下贮藏16d的蓝莓中互交孢霉和灰霉病菌的抑制作用以及对防御相关酶（DREs）活性的诱导作用。结果表明，乙醇蒸汽对绿僵菌菌丝生长的抑制率分别为9.1% （250μlL−1）、36.4% （500μlL−1）和5.5% (1000 μ ll−1)，对绿僵菌菌丝生长的抑制率分别为14.2% （250μlL−1）、44.7% （500μlL−1）和76.6% (1000 μ ll−1)，且呈剂量依赖性。同时，乙醇蒸汽也增强了真菌接种蓝莓DREs的活性，包括β-1,3-葡聚糖酶（GLU）、几丁质酶（CHI）、苯丙氨酸氨解酶（PAL）、过氧化物酶（POD）和多酚氧化酶（PPO）。其中，500μlL−1乙醇蒸汽处理使双生草接种蓝莓的DREs活性分别提高了84.7% （GLU）、88.0% （CHI）、37.9% （PAL）、85.5% （POD）和247.0% (PPO)，使双生草接种蓝莓的DREs活性分别提高了103.8% （GLU）、271.1% （CHI）、41.1% （PAL）、148.3% （POD）和74.4% （PPO）。而在500μl−1乙醇处理的蓝莓中，接种交替芽孢杆菌和灰绿芽孢杆菌，贮藏8d后，PPO活性分别下降了55.2和31.9%。500μl−1乙醇处理蓝莓果实细胞的表面结构和超微结构比其他处理更一体化。本研究结果表明，乙醇可通过激活DREs作为蓝莓对葡萄孢霉和葡萄孢霉腐病的防御反应激活剂，在采后果蔬保鲜中具有实际应用价值。
The aim of the present study was to investigate the effects of ethanol vapor on the inhibition of Alternaria alternata and Botrytis cinerea in postharvest blueberry and the induction of defense-related enzymes (DREs) activities in fungi-inoculated blueberries stored at 0±0.5°C for 16days. Results indicated that ethanol vapor markedly inhibited the mycelial growth of A. alternata and B. cinerea in a dose-dependent manner, with inhibition rates of 9.1% (250μlL−1), 36.4% (500μlL−1), and 5.5% (1,000μlL−1) on A. alternata and 14.2% (250μlL−1), 44.7% (500μlL−1), and 76.6% (1,000μlL−1) on B. cinerea, respectively. Meanwhile, ethanol vapor also enhanced the activities of DREs in fungi-inoculated blueberries, including β-1,3-glucanase (GLU), chitinase (CHI), phenylalnine ammonialyase (PAL), peroxidase (POD), and polyphenol oxidase (PPO). In particular, 500μlL−1 ethanol vapor increased the activities of DREs by 84.7% (GLU), 88.0% (CHI), 37.9% (PAL), 85.5% (POD), and 247.0% (PPO) in A. alternata-inoculated blueberries and 103.8% (GLU), 271.1% (CHI), 41.1% (PAL), 148.3% (POD), and 74.4% (PPO) in B. cinerea-inoculated blueberries, respectively. But, the activity of PPO was decreased by 55.2 and 31.9% in 500μlL−1 ethanol-treated blueberries inoculated with A. alternata and B. cinerea, respectively, after 8days of storage. Moreover, the surface structure and ultrastructure of 500μlL−1 ethanol-treated blueberry fruit cells were more integrated than those of other treatments. The findings of the present study suggest that ethanol could be used as an activator of defense responses in blueberry against Alternaria and Botrytis rots, by activating DREs, having practical application value in the preservation of postharvest fruit and vegetables.
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影响因子：
6.1
作者：
Li, Zhenbiao;Wang, Nan;Shao, Xingfeng
通讯作者：
Shao, Xingfeng
影响因子：
4.1
作者：
Ji, Yaru;Hu, Wenzhong;Feng, Ke
通讯作者：
Feng, Ke
DOI：
10.1080/07060668509501700
发表时间：
1985-01-01
期刊：
CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE
影响因子：
--
作者：
SOMMER, NF
通讯作者：
SOMMER, NF
DOI：
10.1016/j.ijfoodmicro.2010.05.005
发表时间：
2010-08-15
影响因子：
5.4
作者：
Tzortzakis, Nikos G.
通讯作者：
Tzortzakis, Nikos G.
茉莉酸甲酯通过启动防御反应诱导杨梅对柑橘青霉菌的抗性
影响因子：
7
作者：
Wang, Kaituo;Jin, Peng;Zheng, Yonghua
通讯作者：
Zheng, Yonghua