Expression and role of fibroblast activation protein-alpha in microinvasive breast carcinoma.
Expression and role of fibroblast activation protein-alpha in microinvasive breast carcinoma.
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DOI：
10.1186/1746-1596-6-111
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发表时间：
2011-11-08
影响因子：
2.6
通讯作者：
Xian Q
中科院分区：
文献类型：
--
作者：
Hua X;Yu L;Huang X;Liao Z;Xian Q
乳腺导管原位癌（DCIS）的诊断对于病理学家来说是一个挑战，因为原位癌的模式和伪影多种多样，可能被误解为间质浸润。微浸润是通过基底膜和肌上皮界限外存在细胞学恶性细胞来检测的。当恶性细胞侵入间质时，存在由扰动的间质-上皮相互作用诱导的组织重塑。癌相关成纤维细胞（CAFs）是重塑的肿瘤-宿主界面微环境中的主要细胞。它们的特征在于表达特异性成纤维细胞活化蛋白-α（FAP-α），并且不同于表现出CD 34免疫表型的正常成纤维细胞。我们推测FAP-α染色可能有助于确定DCIS是否有微浸润。对349例切除的乳腺标本进行平滑肌肌动蛋白SMA、CD 34、FAP-α和钙调蛋白免疫染色。研究材料分为5组：第1组：整形手术后健康女性的正常乳腺组织;第2组：普通型导管增生（UDH）;第3组：H & E染色无微浸润的DCIS;第4组：H & E染色有微浸润的DCIS（DCIS-MI）;第5组：浸润性导管癌（IDC）。对四种免疫染色剂进行了比较评价。结果表明，FAP-α和Calponin联合应用可使DCIS-MI的病理诊断敏感性提高11.29%，而FAP-α和Calponin联合应用可使DCIS的病理诊断敏感性提高13.6%。本研究首次证明FAP-α和Calponin免疫组化可作为病理诊断DCIS是否存在微浸润的新指标。
Diagnosis of ductal carcinoma in situ (DCIS) in breast cancer cases is challenging for pathologist due to a variety of in situ patterns and artefacts, which could be misinterpreted as stromal invasion. Microinvasion is detected by the presence of cytologically malignant cells outside the confines of the basement membrane and myoepithelium. When malignant cells invade the stroma, there is tissue remodeling induced by perturbed stromal-epithelial interactions. Carcinoma-associated fibroblasts (CAFs) are main cells in the microenvironment of the remodeled tumor-host interface. They are characterized by the expression of the specific fibroblast activation protein-alpha (FAP-α), and differ from that of normal fibroblasts exhibiting an immunophenotype of CD34. We hypothesized that staining for FAP-α may be helpful in determining whether DCIS has microinvasion. 349 excised breast specimens were immunostained for smooth muscle actin SMA, CD34, FAP-α, and Calponin. Study material was divided into 5 groups: group 1: normal mammary tissues of healthy women after plastic surgery; group 2: usual ductal hyperplasia (UDH); group 3: DCIS without microinvasion on H & E stain; group 4: DCIS with microinvasion on H & E stain (DCIS-MI), and group 5: invasive ductal carcinoma (IDC). A comparative evaluation of the four immunostains was conducted. Our results demonstrated that using FAP-α and Calponin adjunctively improved the sensitivity of pathological diagnosis of DCIS-MI by 11.29%, whereas the adjunctive use of FAP-α and Calponin improved the sensitivity of pathological diagnosis of DCIS by 13.6%. This study provides the first evidence that immunostaining with FAP-α and Calponin can serve as a novel marker for pathologically diagnosing whether DCIS has microinvasion.
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影响因子：
4.6
作者：
Dewar, Rajan;Fadare, Oluwole;Gown, Allen M.
通讯作者：
Gown, Allen M.
DOI：
10.1073/pnas.94.12.6307
发表时间：
1997-06-10
影响因子：
11.1
作者：
Chesney, J;Bacher, M;Bucala, R
通讯作者：
Bucala, R
影响因子：
3.4
作者：
Chauhan, H;Abraham, A;Jones, JL
通讯作者：
Jones, JL
影响因子：
50.3
作者：
Allinen, M;Beroukhim, R;Polyak, K
通讯作者：
Polyak, K
影响因子：
30.8
作者：
Hu, M;Yao, J;Polyak, K
通讯作者：
Polyak, K