Proteomics integrated with Escherichia coli vector-based vaccines and antigen microarrays reveals the immunogenicity of a surface sialidase-like protein of Propionibacterium acnes.
Proteomics integrated with Escherichia coli vector-based vaccines and antigen microarrays reveals the immunogenicity of a surface sialidase-like protein of Propionibacterium acnes.
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DOI：
10.1002/prca.200780103
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发表时间：
2008-09
影响因子：
2
通讯作者：
Huang, Chun-Ming
中科院分区：
文献类型：
--
作者：
Huang, Cheng-Po;Liu, Yu-Tsueng;Nakatsuji, Teruaki;Shi, Yang;Gallo, Richard R.;Lin, Shwu-Bin;Huang, Chun-Ming
关键词：
蛋白质组学是鉴定蛋白质的有力工具，为合理设计疫苗提供了依据。然而，利用传统方法检测蛋白质的免疫原性仍然是一项技术性强且耗时的任务。在这里，我们提出了一个有效评估蛋白质免疫原性和抗体检测的平台。破伤风毒素C片段（Tet-c）用作代表性抗原以建立该平台。利用痤疮丙酸杆菌的细胞壁锚定唾液酸酶样蛋白（SLP）来评估该平台的功效。我们通过在大肠杆菌中过表达Tet-c或SLP构建了基于大肠杆菌载体的疫苗。coli中，并利用E.大肠杆菌本身作为疫苗（E. coli Tet-c或SLP载体）。经紫外线照射后，E.将基于大肠杆菌载体的疫苗鼻内给予印迹对照区小鼠，而不加入外源佐剂。对于抗体检测，我们通过用纯化的重组蛋白（包括Tet-c和SLP）打印来制备抗原微阵列。我们的研究结果表明，小鼠鼻内注射紫外线照射的大肠杆菌6周后，可检测到抗体。大肠杆菌载体疫苗。Tet-c和SLP的抗体产生在加强后显著升高。值得注意的是，使用E.大肠杆菌本身作为疫苗载体为旨在筛选新疫苗靶点的应用蛋白质组学提供了关键模板。此外，新的免疫原性SLP潜在地用作开发靶向痤疮丙酸杆菌相关疾病的疫苗的抗原候选物。
Proteomics is a powerful tool for the identification of proteins, which provides a basis for rational vaccine design. However, it is still a highly technical and time-consuming task to examine a protein’s immunogenicity utilizing traditional approaches. Here, we present a platform for effectively evaluating protein immunogenicity and antibody detection. A tetanus toxin C fragment (Tet-c) was used as a representative antigen to establish this platform. A cell wall-anchoring sialidase-like protein (SLP) of Propionibacterium acnes was utilized to assess the efficacy of this platform. We constructed an Escherichia coli vector-based vaccine by overexpressing Tet-c or SLP in E. coli and utilized an intact particle of E. coli itself as a vaccine (E. coli Tet-c or SLP vector). After ultraviolet (UV) irradiation, the E. coli vector-based vaccines were administered intranasally into imprinting control region mice without adding exogenous adjuvants. For antibody detection, we fabricated antigen microarrays by printing with purified recombinant proteins including Tet-c and SLP. Our results demonstrated that detectable antibodies were elicited in mice 6 weeks after intranasal administration of UV-irradiated E. coli vector-based vaccines. The antibody production of Tet-c and SLP was significantly elevated after boosting. Notably, the platform with main benefits of using E. coli itself as a vaccine carrier provides a critical template for applied proteomics aimed at screening novel vaccine targets. In addition, the novel immunogenic SLP potentially serves as an antigen candidate for the development of vaccines targeting P. acnes-associated diseases.
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影响因子：
56.9
作者：
Blander, JM;Medzhitov, R
通讯作者：
Medzhitov, R
影响因子：
82.9
作者：
Brockstedt, DG;Bahjat, KS;Dubensky, TW
通讯作者：
Dubensky, TW
影响因子：
2.2
作者：
FerreiraDaCruz, MDF;GiovannideSimone, S;DanielRibeiro, CT
通讯作者：
DanielRibeiro, CT
影响因子：
3.3
作者：
Garmise, Robert J.;Staats, Herman F.;Hickey, Anthony J.
通讯作者：
Hickey, Anthony J.
影响因子：
56.9
作者：
Brüggemann, H;Henne, A;Gottschalk, G
通讯作者：
Gottschalk, G