Bone marrow processing for transplantation using the COBE spectra cell separator.
Bone marrow processing for transplantation using the COBE spectra cell separator.
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使用 COBE Spectrum 细胞分离器进行骨髓移植处理。
DOI：
10.1089/152581699320207
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
J. Mayer
中科院分区：
文献类型：
--
作者：
Z. Kořístek;J. Mayer
BM 处理、减容以及粒细胞和红细胞去除是尽量减少移植物给药副作用和绕过同种异体 BMT 中 ABO 不相容性的重要考虑因素。我们使用 COBE Spectra 细胞分离器对 33 名患有血液恶性肿瘤和实体瘤的患者（中位年龄 39 岁）进行 BM 处理。我们处理了 42 份 BM 收获物，目的是最大限度地恢复单核细胞 (MNC)。在全身麻醉下从髂后嵴采集BM。过滤前收集的BM的平均体积为1,303ml，收集的总有核细胞(TNC)的平均数量为2.61×10(8)/kg。 BM 处理的平均回收率为 35.8% (10.1%-78.3%) TNC、22.3% (1.1%-75.7%) 粒细胞、78.8% (34.3%-135.2%) MNC、77.2% (8.3%-260.0%) CD34+ 细胞和 153.3%最终产品中的菌落形成单位 (CFU-GM) 为 (32.9%-464.0%)。平均 98.2%（94.5%-99.5%）的红细胞被去除，最终产品中平均有 13.3 毫升（5.1-26.2 毫升）的红细胞。事实证明，使用 COBE Spectra 细胞分离器进行 BM 处理是快速、安全且有效的。然而，收获的CD34+细胞和CFU-GM回收范围非常广泛的原因需要进一步研究。
BM processing, volume reduction, and granulocyte and erythrocyte depletion are important considerations for minimizing the side effects of graft administration and bypassing ABO incompatibility in allogeneic BMT. We used the COBE Spectra cell separator for BM processing in 33 patients suffering from hematologic malignancy and solid tumor (median age 39 years). We processed 42 BM harvests with the aim of maximizing recovery of mononuclear cells (MNC). BM was collected from the posterior iliac crest under general anesthesia. The mean volume of collected BM before filtration was 1,303 ml, and the mean number of collected total nucleated cells (TNC) was 2.61 x 10(8)/kg. The BM processing resulted in a mean recovery of 35.8% (10.1%-78.3%) TNC, 22.3% (1.1%-75.7%) of granulocytes, 78.8% (34.3%-135.2%) of MNC, 77.2% (8.3%-260.0%) of CD34+ cells, and 153.3% (32.9%-464.0%) of colony-forming units (CFU-GM) in the final product. A mean of 98.2% (94.5%-99.5%) of RBC was removed, with a mean of 13.3 ml (5.1-26.2 ml) of RBC in the final product. BM processing using the COBE Spectra cell separator proved to be fast, safe, and effective. However, the reasons for the very wide range of recovery of harvested CD34+ cells and CFU-GM need to be further investigated.
DOI：
10.1089/scd.1.1992.1.233
发表时间：
1992-01-01
期刊：
Journal of hematotherapy
影响因子：
--
作者：
Rowley, S D
通讯作者：
Rowley, S D
影响因子：
2.9
作者：
Lasky,LC;VanBuren,N;Weisdorf,DJ;Filipovich,A;McGlave,P;Kersey,JH;McCullough,J;Ramsay,NK;Blazar,BR
通讯作者：
Blazar,BR
使用 IBM 2991 血细胞处理器通过密度梯度分离制备用于 ABO 不相容骨髓移植的红细胞耗尽骨髓。
影响因子：
2.6
作者：
Jin,NR;Hill,R;Segal,G;Still,B;Petersen,FB;Amos,D;Buckner,CD;Clift,R;Bensinger,W;Martin,P
通讯作者：
Martin,P