Anthelmintic efficacy of 35 herbal medicines against a monogenean parasite, Gyrodactylus kobayashii, infecting goldfish (Carassius auratus)
Anthelmintic efficacy of 35 herbal medicines against a monogenean parasite, Gyrodactylus kobayashii, infecting goldfish (Carassius auratus)
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35 种草药对感染金鱼（Carassius auratus）的单殖寄生虫（Gyrodactylus kobayashii）的驱虫功效
DOI：
10.1016/j.aquaculture.2020.734992
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发表时间：
2020-05
期刊：
影响因子：
4.5
通讯作者：
Ai Xiaohui
中科院分区：
文献类型：
--
作者：
Zhou Shun;Dong Jing;Liu Yongtao;Yang Qiuhong;Xu Ning;Yang Yibin;Gu Zemao;Ai Xiaohui
三旋虫是鱼类常见的单殖体外寄生虫，可给水产养殖业造成严重的经济损失。为寻找有效的、环境可接受的防治水产养殖三代虫病的方法，研究了35种中草药对感染金鱼的小林三代虫的驱蠕虫效果，并采用生物活性导向分离法对有应用前景的中草药进行了进一步分离。在供试的提取物中，重楼甲醇提取物的抗蠕虫活性最高，达100%。kobayashiat 20 mg/L，治疗指数（TI）为37.9，高于水产养殖中广泛使用的基于甲酰胺的杀寄生虫剂。经高效液相色谱分析，鉴定出6个活性成分：薯蓣皂苷、polyphyllin I、gracillin、polyphyllin II、polyphyllin VII和polyphyllin VI，其中薯蓣皂苷对三指虫的驱虫效果最好，其EC 50值为0.17 mg/L，TI值为8.18，在0.5 mg/L时驱虫效果为100%。按有效性顺序，多叶灵I和gracillin的EC 50值分别为0.21和0.19 mg/L，TI值分别为5.35和4.97。表明薯蓣皂苷、多叶薯蓣皂苷I和纤细青霉素治疗G.可能成为水产养殖中抗单殖吸虫治疗剂合成和开发的主要天然化合物。有趣的是，P.与上述活性化合物相比，Polyphylla具有更高的TI值37.9，尽管其需要更高的浓度以达到100%的驱虫功效。结果表明，P. Polyphylla对鱼类的毒性低于上述三种化合物，其驱虫活性虽然不如上述三种化合物，但其甲醇提取物是一种相对安全和有益的药物，可用于实际应用于治疗水产养殖中的三指藻病。
Species ofGyrodactylusare common monogenean ectoparasites of fish and can result in severe economic losses in aquaculture. To find effective and environmentally acceptable therapies to control gyrodactylosis in aquaculture, anthelmintic efficacy of 35 kinds of herbal medicines was investigated againstGyrodactylus kobayashiiinfecting goldfish (Carassius auratus), and the promising herbal medicines were further isolated by bioassay-guided fractionation. Among the tested extracts, the methanol extract ofParispolyphyllawas the most effective and showed 100% anthelmintic activity againstG. kobayashiiat 20 mg/L, and had a therapeutic index (TI) of 37.9, which is higher than that of widely used formaldehyde-based parasiticides in aquaculture. According to high-performance liquid chromatography (HPLC) analysis, six active compounds were identified: dioscin, polyphyllin I, gracillin, polyphyllin II, polyphyllin VII and polyphyllin VI; among these active compounds, dioscin was the most effective in eliminatingGyrodactylusinfections, with EC50value of 0.17 mg/L and TI value of 8.18, and exhibiting 100% anthelmintic efficacy at 0.5 mg/L. In order of efficacy, polyphyllin I and gracillin had EC50values of 0.21 and 0.19 mg/L and TI values of 5.35 and 4.97, respectively. These findings suggested that dioscin, polyphyllin I and gracillin are effective and safe in the treatment ofG. kobayashiiinfections and might become leading natural compounds for the synthesis and development of therapeutic agents against monogenean parasites in aquaculture. Interestingly, the methanol extract ofP. polyphyllahad a higher TI value of 37.9 compared to the abovementioned active compounds, although it required higher concentrations to reach 100% anthelmintic efficacy. The results indicated the methanol extract ofP. polyphyllawas less toxic to fish than the three compounds mentioned above, and although its anthelmintic activity was inferior to that of these compounds, the methanol extract was a relatively safe and beneficial agent for practical application to treat gyrodactylosis in aquaculture.
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影响因子：
2.4
作者：
Cable, J;Tinsley, RC;Harris, PD
通讯作者：
Harris, PD
影响因子：
7.9
作者：
Wang, G. -X.;Han, J.;Liu, X. -L.
通讯作者：
Liu, X. -L.
影响因子：
2.6
作者：
Fridman, S.;Sinai, T.;Zilberg, D.
通讯作者：
Zilberg, D.
DOI：
--
发表时间：
2008
期刊：
--
影响因子：
--
作者：
T. Mo;Annette Kristensen;K. Norheim;P. Jansen
通讯作者：
T. Mo;Annette Kristensen;K. Norheim;P. Jansen
影响因子：
2.4
作者：
Cable, J;Harris, PD;Bakke, TA
通讯作者：
Bakke, TA