The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model.
The effect of platelet-rich plasma on Achilles tendon healing in a rabbit model.
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DOI：
10.1016/j.aott.2016.12.001
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发表时间：
2017-01
影响因子：
1
通讯作者：
Neo M
中科院分区：
文献类型：
--
作者：
Takamura M;Yasuda T;Nakano A;Shima H;Neo M
本研究的目的是通过组织学检查和定量评估PRP对兔跟腱炎症、增殖和重塑阶段愈合的影响。50只成年雄性日本白化兔跟腱断裂，随机分为两组，分别给予富血小板血浆（PRP）治疗和不给予治疗。在治疗后1、2、3、4和6周采集肌腱组织，切片用苏木精-伊红和抗CD31和I型胶原的单克隆抗体染色。与对照组相比，PRP组胶原纤维在断裂后早期增殖更密集，随后胶原纤维的重塑和向正常肌腱组织的近似发生得更早。1、2周时，PRP组成纤维细胞数量明显高于对照组。同样，在第1周和第2周，PRP组cd31阳性细胞的面积比明显高于对照组。3周后，PRP组I型胶原阳性染色比对照组更强烈，表明肌腱成熟。PRP可缩短炎症期，促进肌腱增生期愈合。
The aim of the present study was to evaluate the effects of PRP on Achilles tendon healing in rabbits during the inflammatory, proliferative, and remodeling phases by histological examination and quantitative assessments. Fifty mature male Japanese albino rabbits with severed Achilles tendons were divided into two equal groups and treated with platelet-rich plasma (PRP) or left untreated. Tendon tissue was harvested at 1, 2, 3, 4, and 6 weeks after treatment, and sections were stained with hematoxylin-eosin and monoclonal antibodies against CD31 and type I collagen. Collagen fibers proliferated more densely early after severance, and subsequent remodeling of the collagen fibers and approximation of normal tendinous tissue occurred earlier in the PRP group than in the control group. The fibroblast number was significantly higher in the PRP group than in the control group at 1 and 2 weeks. Similarly, the area ratio of CD31-positive cells was significantly higher in the PRP group than in the control group at 1 and 2 weeks. Positive staining for type I collagen was more intense in the PRP group than in the control group after 3 weeks, indicating tendon maturation. Administration of PRP shortened the inflammatory phase and promoted tendon healing during the proliferative phase.
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影响因子：
4.8
作者：
Schepull, Thorsten;Kvist, Joanna;Aspenberg, Per
通讯作者：
Aspenberg, Per
DOI：
10.1016/j.fas.2009.09.002
发表时间：
2010-09-01
期刊：
Foot and ankle surgery : official journal of the European Society of Foot and Ankle Surgeons
影响因子：
--
作者：
Lyras, Dimitrios N;Kazakos, Konstantinos;Verettas, Dionysios
通讯作者：
Verettas, Dionysios
DOI：
10.1080/00016470410001708190
发表时间：
2004-02-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
Aspenberg, P;Virchenko, O
通讯作者：
Virchenko, O
影响因子：
19.7
作者：
Fukawa, Taisuke;Yamaguchi, Satoshi;Takahashi, Kazuhisa
通讯作者：
Takahashi, Kazuhisa
影响因子：
4.8
作者：
Metz, Roderick;Verleisdonk, Egbert-Jan M. M.;van der Werken, Christiaan
通讯作者：
van der Werken, Christiaan