Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.
Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.
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DOI：
10.1084/jem.152.6.1709
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发表时间：
1980-12-01
期刊：
影响因子：
--
通讯作者：
Watson J
中科院分区：
文献类型：
--
作者：
Gillis S;Watson J
为了筛选出稳定产生人白细胞介素2(IL-2；以前称为T细胞生长因子)的肿瘤细胞系，对16株人T、B白血病细胞系进行了结构性和有丝分裂原刺激产生IL-2的筛选。我们发现名为Jurkat-FHCRC的T细胞白血病株在T细胞有丝分裂原刺激24小时后产生200U/ml的IL-2活性。在用1%植物血凝素或20微克/毫升刀豆球蛋白刺激24小时的Jurkat-FHCRC细胞培养上清液中，发现有丝分裂原诱导的IL-2活性最高。在有丝分裂原刺激的培养物中加入脂肪酸衍生物佛波酯，可使Jurkat-FHCRC细胞产生的IL-2浓度增加到400U/ml。在这种情况下观察到的IL-2活性是有丝分裂原或同种异体抗原刺激的正常人外周血或脾淋巴细胞常规培养的100-300倍。Jurkat-FHCRC来源的条件培养液在体外对小鼠和人激活的T细胞株的持续增殖显示出同等的能力，证实了Jurkat-FHCRC细胞产生人IL-2的能力。这些研究确定了人IL-2的新来源，并为分离和进一步鉴定这种免疫调节分子建立了有价值的试剂。
To isolate a stable tumor cell line capable of producing human interleukin 2 (IL-2; formerly referred to as T cell growth factor), 16 human T and B leukemia cell lines were screened for constitutive and mitogen-stimulated IL-2 production. We found that the T cell leukemia line designated Jurkat-FHCRC produced > 200 U/ml of IL-2 activity after a 24-h stimulation with T cell mitogens. Peak mitogen-induced IL-2 activity was found in supernates harvested from 24-h Jurkat-FHCRC cell cultures stimulated with either 1% phytohemagglutinin or 20 microgram/ml concanavalin A. Addition of the fatty acid derivative phorbol myristate acetate to mitogen-stimulated cultures increased Jurkat-FHCRC IL-2 production to concentrations > 400 U/ml. IL-2 activity observed in such cases represented between 100--300 times that produced in conventional cultures of mitogen- or alloantigen-stimulated normal human peripheral blood or splenic lymphocytes. Jurkat-FHCRC- derived conditioned medium demonstrated equal capacity to promote the sustained in vitro proliferation of either murine or human activated T cell lines confirming the ability of Jurkat-FHCRC cells to produce human IL-2. These studies identify a new source of human IL-2 and establish a valuable reagent for the isolation and further molecular characterization of this immunoregulatory molecule.
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DOI：
10.1084/jem.148.4.1093
发表时间：
1978-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gillis S;Baker PE;Ruscetti FW;Smith KA
通讯作者：
Smith KA
鼠抗原特异性辅助 T 淋巴细胞在培养物中持续增殖。
影响因子：
15.3
作者：
Watson, J
通讯作者：
Watson, J
影响因子：
64.8
作者：
GILLIS, S;SMITH, KA
通讯作者：
SMITH, KA
影响因子：
64.8
作者：
LARSSON, EL;ISCOVE, NN;COUTINHO, A
通讯作者：
COUTINHO, A
影响因子：
15.3
作者：
WATSON, J;GILLIS, S;SMITH, KA
通讯作者：
SMITH, KA