Cellular distribution of chromogranin A in excitatory, inhibitory, aminergic and peptidergic neurons of the rodent central nervous system.
Cellular distribution of chromogranin A in excitatory, inhibitory, aminergic and peptidergic neurons of the rodent central nervous system.
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DOI：
10.1016/j.regpep.2009.11.021
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发表时间：
2010-11-30
影响因子：
--
通讯作者：
Weihe, E.
中科院分区：
文献类型：
--
作者：
Schafer, M. K. -H.;Mahata, S. K.;Stroth, N.;Eiden, L. E.;Weihe, E.
使用识别WE14表位的抗体，在啮齿动物中枢神经系统中解剖学定义的GABA能、胆碱能、胆碱能、儿茶酚胺能和肽能细胞群的末端区域和细胞体中，检查了嗜铬粒蛋白A（CGA）的加工和未加工形式的免疫反应性。CGA普遍存在于神经元细胞体内，没有明显的解剖学或化学编码的神经系统亚群，其中CGA在大多数神经元中不表达。CGA的表达是基本上没有从在中枢神经系统中的儿茶酚胺能终端领域，表明一个相对缺乏的大致密核心囊泡在中枢神经系统相比，外周的儿茶酚胺能神经元。广泛的突触共定位与经典的递质标记物没有观察到，即使在杏仁核，其中CGA纤维是众多的领域，这表明优先隔离的CGA肽能终端在中枢神经系统。CGA在中枢神经系统某些区域的树突中的定位可能表明其参与调节树突释放机制。最后，CGA在神经元细胞胞体中的普遍存在，特别是在GABA能神经元中的显著存在，表明CGA在CNS中的第二种非分泌囊泡相关功能。我们建议，CGA可能在中枢神经系统中作为激素原和颗粒形成因子在某些终端领域，但也具有尚未未知的独特的细胞功能内的神经元胞体和树突。
Immunoreactivity for both processed and unprocessed forms of chromogranin A (CGA) was examined, using an antibody recognizing the WE14 epitope, among terminal fields and cell bodies of anatomically defined GABAergic, glutamatergic, cholinergic, catecholaminergic, and peptidergic cell groups in the rodent central nervous system. CGA is ubiquitous within neuronal cell bodies, with no obvious anatomical or chemically-coded subdivision of the nervous system in which CGA is not expressed in most neurons. CGA expression is essentially absent from catecholaminergic terminal fields in the CNS, suggesting a relative paucity of large dense-core vesicles in CNS compared to peripheral catecholaminergic neurons. Extensive synaptic co-localization with classical transmitter markers is not observed even in areas such as amygdala, where CGA fibers are numerous, suggesting preferential segregation of CGA to peptidergic terminals in CNS. Localization of CGA in dendrites in some areas of CNS may indicate its involvement in regulation of dendritic release mechanisms. Finally, the ubiquitous presence of CGA in neuronal cell somata, especially pronounced in GABAergic neurons, suggests a second non-secretory vesicle-associated function for CGA in CNS. We propose that CGA may function in the CNS as a prohormone and granulogenic factor in some terminal fields, but also possesses as-yet unknown unique cellular functions within neuronal somata and dendrites.
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影响因子：
3
作者：
Beinfeld MC;Funkelstein L;Foulon T;Cadel S;Kitagawa K;Toneff T;Reinheckel T;Peters C;Hook V
通讯作者：
Hook V
影响因子：
64.8
作者：
IACANGELO, A;AFFOLTER, HU;GRIMES, M
通讯作者：
GRIMES, M
DOI：
10.1073/pnas.79.19.6056
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
COHN, DV;ZANGERLE, R;WINKLER, H
通讯作者：
WINKLER, H
影响因子：
15.9
作者：
Mahata, SK;OConnor, DT;Parmer, RJ
通讯作者：
Parmer, RJ
影响因子：
--
作者：
Mahata, SK;Mahata, M;O'Connor, DT
通讯作者：
O'Connor, DT