Meta-analyses of studies of the human microbiota.
Meta-analyses of studies of the human microbiota.
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DOI：
10.1101/gr.151803.112
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发表时间：
2013-10
期刊：
影响因子：
7
通讯作者：
Knight R
中科院分区：
文献类型：
--
作者：
Lozupone CA;Stombaugh J;Gonzalez A;Ackermann G;Wendel D;Vázquez-Baeza Y;Jansson JK;Gordon JI;Knight R
我们的身体栖息地相关的微生物群落因其对人类健康的影响而引起了广泛的研究兴趣。由于许多微生物群研究都是基于相同的细菌 16S 核糖体 RNA (rRNA) 基因靶标，因此原则上可以对它们进行比较，以确定不同疾病/生理/发育状态的相对重要性。然而，所使用的实验方案的差异可能会产生超过生物学差异的变异。通过使用 QIIME 数据库对人类微生物群的多项研究生成的 16S rRNA 基因序列进行比较，我们发现不同身体部位的微生物群组成的差异始终大于研究中的技术差异。然而，来自西方成人粪便微生物群不同研究的样本通常按研究聚集，并且 16S rRNA 目标区域、DNA 提取技术和测序平台在观察到的多样性中产生系统偏差，可能掩盖具有生物学意义的成分差异。相比之下，随着年龄的增长以及西方文化和农业文化之间的粪便微生物群的系统组成差异，足以超过技术差异。此外，患有回肠克罗恩病和妊娠晚期的个体通常比来自同一研究的对照组更类似于来自不同研究的婴儿，这表明这些不同的发育/生理/疾病状态具有平行的组成属性。总之，这些结果表明，当研究的参数具有较大的效应量时，人类微生物群的交叉研究比较是有价值的，但研究对人类微生物群更微妙的影响需要仔细选择对照群体和标准化方案。
Our body habitat-associated microbial communities are of intense research interest because of their influence on human health. Because many studies of the microbiota are based on the same bacterial 16S ribosomal RNA (rRNA) gene target, they can, in principle, be compared to determine the relative importance of different disease/physiologic/developmental states. However, differences in experimental protocols used may produce variation that outweighs biological differences. By comparing 16S rRNA gene sequences generated from diverse studies of the human microbiota using the QIIME database, we found that variation in composition of the microbiota across different body sites was consistently larger than technical variability across studies. However, samples from different studies of the Western adult fecal microbiota generally clustered by study, and the 16S rRNA target region, DNA extraction technique, and sequencing platform produced systematic biases in observed diversity that could obscure biologically meaningful compositional differences. In contrast, systematic compositional differences in the fecal microbiota that occurred with age and between Western and more agrarian cultures were great enough to outweigh technical variation. Furthermore, individuals with ileal Crohn's disease and in their third trimester of pregnancy often resembled infants from different studies more than controls from the same study, indicating parallel compositional attributes of these distinct developmental/physiological/disease states. Together, these results show that cross-study comparisons of human microbiota are valuable when the studied parameter has a large effect size, but studies of more subtle effects on the human microbiota require carefully selected control populations and standardized protocols.
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影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
De Vos W
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
2.5
作者：
Huurre, Anu;Kalliomaki, Marko;Isolauri, Erika
通讯作者：
Isolauri, Erika
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI