Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis.
Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis.
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发现磷脂酸、磷脂酰胆碱和磷脂酰丝氨酸作为子宫内膜异位症早期诊断的生物标志物
DOI：
10.3389/fphys.2018.00014
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发表时间：
2018
影响因子：
4
通讯作者：
Bi H
中科院分区：
文献类型：
--
作者：
Li J;Gao Y;Guan L;Zhang H;Sun J;Gong X;Li D;Chen P;Ma Z;Liang X;Huang M;Bi H
子宫内膜异位症的早期临床诊断指标和无创性诊断方法的敏感性和特异性均不理想。以往研究表明，脂质代谢异常参与了子宫内膜异位症的病理发展。我们的横断面研究包括2014年1月至2015年1月期间接受诊断性腹腔镜联合宫腔镜检查的21例腹腔镜证实的I-II期子宫内膜异位症患者和20例不孕妇女。通过子宫内膜活检收集在位子宫内膜。通过超高效液相色谱-电喷雾电离高分辨率质谱（UHPLC-ESI-HRMS）定量脂质代谢产物。与对照组相比，子宫内膜异位症早期（I-II）患者的血脂特征为磷脂酰胆碱（18：1/22：6）、（20：1/14：1）、（20：3/20：4）和磷脂酰丝氨酸（20：3/23：1）浓度降低，磷脂酸（25：5/22：6）浓度升高。5种生物标志物的综合预测策略的特异性为75.0%，敏感性为90.5%。基于UHPLC-ESI-HRMS的代谢组学有效地表征了子宫内膜异位症在位子宫内膜的脂质谱。本研究揭示了磷脂酸、磷脂酰胆碱、磷脂酰丝氨酸代谢产物在子宫内膜异位症中的变化，为子宫内膜异位症的早期半侵入性诊断提供了潜在的生物标志物。
The sensitivity and specificity of clinical diagnostic indicators and non-invasive diagnostic methods for endometriosis at early stage is not optimal. Previous studies demonstrated that abnormal lipid metabolism was involved in the pathological development of endometriosis. Our cross-sectional study included 21 patients with laparoscopically confirmed endometriosis at stage I–II and 20 infertile women who underwent diagnostic laparoscopy combined with hysteroscopy from January 2014 to January 2015. Eutopic endometrium was collected by pipelle endometrial biopsy. Lipid metabolites were quantified by ultra-high performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (UHPLC-ESI-HRMS). Lipid profiles of endometriosis patients at early stage (I–II) was characterized by a decreased concentration of phosphatidylcholine (18:1/22:6), (20:1/14:1), (20:3/20:4), and phosphatidylserine (20:3/23:1) and an increased concentration of phosphatidic acid (25:5/22:6) compared with control. The synthesized predicting strategy with 5 biomarkers has a specificity of 75.0% and a sensitivity of 90.5%. Lipid profile of eutopic endometrium in endometriosis was effectively characterized by UHPLC-ESI-HRMS-based metabolomics. Our study demonstrated the alteration of phosphatidic acid, phosphatidylcholine, phosphatidylserine metabolites in endometriosis and provided potential biomarkers for semi-invasive diagnose of endometriosis at early stage.
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大规模代谢组分析确定了出现冠心病的新生物标志物。
影响因子：
4.5
作者：
Ganna A;Salihovic S;Sundström J;Broeckling CD;Hedman AK;Magnusson PK;Pedersen NL;Larsson A;Siegbahn A;Zilmer M;Prenni J;Arnlöv J;Lind L;Fall T;Ingelsson E
通讯作者：
Ingelsson E
影响因子：
4.6
作者：
Chagovets VV;Wang Z;Kononikhin AS;Starodubtseva NL;Borisova A;Salimova D;Popov IA;Kozachenko AV;Chingin K;Chen H;Frankevich VE;Adamyan LV;Sukhikh GT
通讯作者：
Sukhikh GT
DOI：
10.1016/s0889-8545(05)70302-8
发表时间：
1997-06-01
影响因子：
3.2
作者：
Eskenazi, B;Warner, ML
通讯作者：
Warner, ML
影响因子：
4.4
作者：
Dutta, Mainak;Anitha, Mallappa;Patterson, Andrew D.
通讯作者：
Patterson, Andrew D.
影响因子：
6.1
作者：
Dunselman, G. A. J.;Vermeulen, N.;Nelen, W.
通讯作者：
Nelen, W.