Secretory peptide PdEPF2 enhances drought tolerance by modulating stomatal density and regulates ABA response in transgenic Arabidopsis thaliana
Secretory peptide PdEPF2 enhances drought tolerance by modulating stomatal density and regulates ABA response in transgenic Arabidopsis thaliana
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分泌肽 PdEPF2 通过调节气孔密度增强耐旱性并调节转基因拟南芥的 ABA 反应
DOI：
10.1007/s11240-016-0957-x
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发表时间：
2016-02
影响因子：
3
通讯作者：
Yin, Weilun
中科院分区：
文献类型：
--
作者：
An, Yi;Liu, Chao;Xia, Xinli;Yin, Weilun
缺水限制了全世界植物的生长和生产力。提高水利用效率（WUE）和耐旱性是解决这些限制的重要适应措施。本研究从速生杨树无性系NE-19 （Populus nigra × （Populus deltoids × Populus nigra））中分离到一个表皮图案因子PdEPF2。定量反转录聚合酶链反应表明，该基因的转录受干旱和ABA诱导。为了研究PdEPF2的生物学功能，我们构建了PdEPF2组成表达基因（35S:PdEPF2）的转基因拟南芥植株。与野生型和epf2-3突变体相比，异位表达PdEPF2的转基因植株在干旱胁迫下的种子发芽率、初生根长、脯氨酸和叶绿素含量、Fv/Fm、光合速率和叶片瞬时水分利用效率等渗透参数均表现出较好的优势。此外，由于气孔密度降低，转基因拟南芥植株的耐旱性增强，从而限制了蒸腾作用，减少了水分流失。与野生型相比，过表达PdEPF2的植株在萌发和幼苗发育过程中对外源ABA的敏感性降低，而epf2-3突变体对外源ABA的敏感性增加。此外，PdEPF2正调控ABA信号相关基因ABI1和ABI2的表达。这些结果表明，PdEPF2可能通过调节气孔密度和对ABA信号通路的响应来增强抗旱性。
Water deficit limits the growth and productivity of plants worldwide. Improved water use efficiency (WUE) and drought tolerance are important adaptations to address these limitations. In this study, an epidermal patterning factor (EPF), PdEPF2, from a fast-growing poplar clone NE-19 (Populus nigra × (Populus deltoids × Populus nigra)) was isolated. Quantitative reverse transcription polymerase chain reaction showed that transcription of this gene was induced by drought and abscisic acid (ABA). To study the biological functions of PdEPF2, transgenic Arabidopsis plants harboring (35S:PdEPF2) in which PdEPF2 was constitutively expressed were generated. Compared with the wild type and epf2-3 mutant, the transgenic plants ectopically expressing PdEPF2 showed favorable osmotic parameters, such as seed germination rate, primary root length, proline and chlorophyll content, Fv/Fm, photosynthetic rate, and instantaneous leaf WUE, under drought stress. In addition, the transgenic Arabidopsis plants displayed enhanced drought tolerance as a result of decreased stomatal density, which would limit transpiration and reduce water loss. Compared with the wild-type, plants that overexpressed PdEPF2 had decreased sensitivity to exogenous ABA during germination and seedling development, whereas the epf2-3 mutant showed increased sensitivity to ABA. Furthermore, PdEPF2 positively regulated expression of two ABA signaling-related genes, ABI1 and ABI2. These findings indicate that PdEPF2 may enhance drought tolerance by regulating stomatal density and the response to the ABA signaling pathway.
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