Mild hydrostatic pressure triggers oxidative responses in Escherichia coli.
Mild hydrostatic pressure triggers oxidative responses in Escherichia coli.
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DOI：
10.1371/journal.pone.0200660
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Guyet A;Dade-Robertson M;Wipat A;Casement J;Smith W;Mitrani H;Zhang M
静液压是一种重要的物理刺激，可以在细菌细胞中引起各种反应。已经研究了大肠杆菌在10 MPa至110 MPa之间的大肠杆菌的存活和细胞过程。但是，了解对中度升高压力高达10 MPa的细菌反应对于包括智能和响应材料（包括智能和响应材料）的一系列不同应用是有用的。在这项研究中，使用RNA-Seq的转录组分析检查了大肠杆菌K-12 mg1655至1 MPa压力的遗传反应。结果表明，在大肠杆菌细胞中，在1 MPa压力下差异表达了101个基因，其中85个上调了。分析表明，某些基因过度表达以适应我们系统中氧气水平的增加，并且涉及几种功能类别，包括氧化应激反应，FE-S簇组装和铁的获取。使用RT-QPCR进一步研究了两个差异表达的基因AZUC和ENTC，GFP报道了这两个基因的菌株AG1319（Pazuc AZUC AZUC-MSFGFP）和AG1321和AG1321（PENTC ENTC ENTC-MSFGFP）。通过监测菌株AG1319（Pazuc AZUC AZUC-MSFGFP）的荧光信号，观察到AZUC表达的线性反应在0 MPa至1 MPa之间。这项研究是第一个证明细菌细胞在1 MPA静液压压力下的遗传反应的报告，并提供了用于在广泛应用中创建压力传感细菌菌株的初步数据。
Hydrostatic pressure is an important physical stimulus which can cause various responses in bacterial cells. The survival and cellular processes of Escherichia coli under hydrostatic pressures between 10 MPa and 110 MPa have been studied. However, understanding bacterial responses to moderately elevated pressure of up to 10 MPa is useful for a range of different applications including for example in smart and responsive materials. In this study, the genetic responses of E. coli K-12 MG1655 to 1 MPa pressure was examined using transcriptomic analysis by RNA-Seq. The results show that 101 genes were differentially expressed under 1 MPa pressure in E. coli cells, with 85 of them up-regulated. The analysis suggested that some genes were over expressed to adapt the increase of oxygen levels in our system, and several functional categories are involved including oxidative stress responses, Fe-S cluster assembly and iron acquisition. Two differentially expressed genes azuC and entC were further investigated using RT-qPCR, and GFP reported strains of those two genes were created, AG1319 (PazuC azuC-msfgfp) and AG1321 (PentC entC-msfgfp). A linear response of azuC expression was observed between 0 MPa to 1 MPa by monitoring the fluorescence signal of strain AG1319 (PazuC azuC-msfgfp). This study is the first report to demonstrate the genetic response of bacterial cells under 1 MPa hydrostatic pressure, and provides preliminary data for creating pressure sensing bacterial strains for a wide range of applications.
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影响因子：
6.4
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