A novel salicylaldehyde dehydrogenase-NahV involved in catabolism of naphthalene from Pseudomonas putida ND6
A novel salicylaldehyde dehydrogenase-NahV involved in catabolism of naphthalene from Pseudomonas putida ND6
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一种新型水杨醛脱氢酶-NahV参与恶臭假单胞菌ND6萘的分解代谢
DOI：
10.1007/s11434-007-0296-8
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发表时间：
2007-07
期刊：
影响因子：
--
通讯作者：
Li, YongJun
中科院分区：
文献类型：
--
作者：
Zhao, HuaBing;Cai, BaoLi;Chen, Wei;Li, YongJun
一种新的参与恶臭假单胞菌ND6 （NahV）萘分解代谢的水杨醛脱氢酶被发现。NahV在氨基酸序列上与P. putida ND6的经典水杨醛脱氢酶NahF具有较低的同源性。这是首次报道的细菌水杨醛脱氢酶的同功能酶。这两种酶都具有广泛的底物特异性，并能催化水杨醛、5-氯水杨醛、甲醛、间硝基苯甲醛、邻硝基苯甲醛、邻甲氧基苯甲醛、戊二醛、癸醛和乙二醛的氧化。然而，所取代的类似物转化的相对速率差别很大。Fe2+、Cu2+和Zn2+均能增强NahV活性；而NahF活性只能由Fe2+激发。NahF在高温下比NahV更稳定。斑点杂交分析表明，本研究分离的所有萘降解菌中均存在nahf样基因，而仅在部分萘降解菌中存在nahv样基因。
A novel salicylaldehyde dehydrogenase involved in catabolism of naphthalene from Pseudomonas putida ND6, NahV, has been identified. NahV exhibited lower identity in amino acid sequence with the classic salicylaldehyde dehydrogenase, NahF, from P. putida ND6. This is the first report of an isofunctional enzyme of bacterial salicylaldehyde dehydrogenase. Both enzymes exhibited broad substrate specificities and catalyzed the oxidation of salicylaldehyde, 5-chlorosalicylaldehyde, formaldehyde, m-nitrobenzaldehyde, o-nitrobenzaldehyde, o-methoxybenxaldehyde, glutaraldehyde, caprylic aldehyde, and glyoxal. However, the relative rates at which the substituted analogs are transformed differ considerably. NahV activity could be enhanced by Fe2+, Cu2+ and Zn2+; whereas NahF activity could only be stimulated by Fe2+. NahF is more stable than NahV at elevated temperatures. Dot-blot hybridization analyses showed that nahF-like genes occurred in all naphthalene-degradation bacteria isolated in this study, whereas nahV-like genes were present in only some naphthalene-degrading bacteria.
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影响因子：
3.2
作者：
Sota, M;Yano, H;Tsuda, M
通讯作者：
Tsuda, M
DOI：
--
发表时间：
2001-01
期刊：
--
影响因子：
--
作者：
J. Sambrook;E. Fritsch;T. Maniatis
通讯作者：
J. Sambrook;E. Fritsch;T. Maniatis
影响因子：
4.4
作者：
Jeon, CO;Park, M;Madsen, EL
通讯作者：
Madsen, EL
影响因子：
3.2
作者：
Caroline M. Laemmli;J. Leveau;A. Zehnder;J. R. van der Meer
通讯作者：
Caroline M. Laemmli;J. Leveau;A. Zehnder;J. R. van der Meer
影响因子：
3.5
作者：
Bosch, R;García-Valdés, E;Moore, ERB
通讯作者：
Moore, ERB