Tracking Pathogen Infections by Time-Resolved Chemical Proteomics.
Tracking Pathogen Infections by Time-Resolved Chemical Proteomics.
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DOI：
10.1002/anie.201911078
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发表时间：
2020-02-03
期刊：
影响因子：
--
通讯作者：
Tao WA
中科院分区：
文献类型：
--
作者：
Zhang Y;Kao DS;Gu B;Bomjan R;Srivastava M;Lu H;Zhou D;Tao WA
Studying the dynamic interaction between host cells and pathogen is vital but remains technically challenging. We describe herein a time-resolved chemical proteomics strategy enabling host and pathogen temporal interaction profiling (HAPTIP) for tracking the entry of a pathogen into the host cell. A novel multifunctional chemical proteomics probe was introduced to label living bacteria followed by in vivo crosslinking of bacteria proteins to their interacting host-cell proteins at different time points initiated by UV for label-free quantitative proteomics analysis. We observed over 400 specific interacting proteins crosslinked with the probe during the formation of Salmonella-containing vacuole (SCV). This novel chemical proteomics approach provides a temporal interaction profile of host and pathogen in high throughput and would facilitate better understanding of the infection process at the molecular level.
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