The mechanical properties and cytotoxicity of cell-laden double-network hydrogels based on photocrosslinkable gelatin and gellan gum biomacromolecules.
The mechanical properties and cytotoxicity of cell-laden double-network hydrogels based on photocrosslinkable gelatin and gellan gum biomacromolecules.
复制标题
DOI：
10.1016/j.biomaterials.2011.12.050
复制
发表时间：
2012-04
期刊：
影响因子：
14
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Shin, Hyeongho;Olsen, Bradley D.;Khademhosseini, Ali
水凝胶应用于组织工程支架，特别是软骨等承载组织的一个主要目标是开发具有高机械强度的水凝胶。在这项研究中，采用双网络（DN）策略来设计可以封装细胞的强水凝胶。我们通过使用与细胞存活相容的加工条件对之前研究的双网络（DN）水凝胶进行了改进。 DN 水凝胶是通过两步光交联创建的，使用甲基丙烯酸结冷胶 (GGMA) 作为刚性和脆性的第一网络，使用明胶甲基丙烯酰胺 (GelMA) 作为柔软和延展的第二网络。我们控制每种聚合物的甲基丙烯酸化程度，以便它们获得与每种网络相关的机械性能。 DN是通过光交联GGMA、将GelMA扩散到第一网络中以及光交联GelMA形成第二网络而形成的。通过将大 GelMA 分子扩散到 GGMA 网络中的扩散测试、由此产生的机械性能增强以及 GGMA/GelMA 单一网络 (SN) 和 DN 之间机械性能的差异来检查 DN 的形成。所得的 DN 水凝胶表现出高达 6.9 MPa 的压缩破坏应力，接近软骨的强度。研究发现，对于高强度的 DN 水凝胶来说，第二网络的交联密度存在一个最佳范围。 GelMA 与 GGMA 质量比较高的 DN 水凝胶表现出更高的强度，这显示出未来开发更强的 DN 水凝胶的前景。 NIH-3T3 成纤维细胞的三维 (3D) 封装和随后的活力测试显示了 DN 形成过程的细胞相容性。由于具有高强度和封装细胞的能力，由可光交联大分子制成的 DN 水凝胶可用于承重组织的再生。
A major goal in the application of hydrogels for tissue engineering scaffolds, especially for load-bearing tissues such as cartilage, is to develop hydrogels with high mechanical strength. In this study, a double-network (DN) strategy was used to engineer strong hydrogels that can encapsulate cells. We improved upon previously studied double-network (DN) hydrogels by using a processing condition compatible with cell survival. The DN hydrogels were created by a two-step photocrosslinking using gellan gum methacrylate (GGMA) for the rigid and brittle first network, and gelatin methacrylamide (GelMA) for the soft and ductile second network. We controlled the degree of methacrylation of each polymer so that they obtain relevant mechanical properties as each network. The DN was formed by photocrosslinking the GGMA, diffusing GelMA into the first network, and photocrosslinking the GelMA to form the second network. The formation of the DN was examined by diffusion tests of the large GelMA molecules into the GGMA network, the resulting enhancement in the mechanical properties, and the difference in mechanical properties between GGMA/GelMA single networks (SN) and DNs. The resulting DN hydrogels exhibited the compressive failure stress of up to 6.9 MPa, which approaches the strength of cartilage. It was found that there is an optimal range of the crosslink density of the second network for high strength of DN hydrogels. DN hydrogels with a higher mass ratio of GelMA to GGMA exhibited higher strength, which shows promise in developing even stronger DN hydrogels in the future. Three dimensional (3D) encapsulation of NIH-3T3 fibroblasts and the following viability test showed the cell-compatibility of the DN formation process. Given the high strength and the ability to encapsulate cells, the DN hydrogels made from photocrosslinkable macromolecules could be useful for the regeneration of load-bearing tissues.
登录
查看更多内容
影响因子：
11.2
作者：
Hamcerencu, Mihaela;Desbrieres, J.;Riess, G.
通讯作者：
Riess, G.
影响因子：
--
作者：
Brigham MD;Bick A;Lo E;Bendali A;Burdick JA;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
3.6
作者：
Bryant, SJ;Nuttelman, CR;Anseth, KS
通讯作者：
Anseth, KS
影响因子：
29.4
作者：
Gong, JP;Katsuyama, Y;Osada, Y
通讯作者：
Osada, Y
影响因子：
14
作者：
Coutinho, Daniela F.;Sant, Shilpa V.;Shin, Hyeongho;Oliveira, Joao T.;Gomes, Manuela E.;Neves, Nuno M.;Khademhosseini, Ali;Reis, Rui L.
通讯作者：
Reis, Rui L.