Modified Gellan Gum hydrogels with tunable physical and mechanical properties.
Modified Gellan Gum hydrogels with tunable physical and mechanical properties.
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DOI：
10.1016/j.biomaterials.2010.06.035
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发表时间：
2010-10
期刊：
影响因子：
14
通讯作者：
Reis, Rui L.
中科院分区：
文献类型：
--
作者：
Coutinho, Daniela F.;Sant, Shilpa V.;Shin, Hyeongho;Oliveira, Joao T.;Gomes, Manuela E.;Neves, Nuno M.;Khademhosseini, Ali;Reis, Rui L.
关键词：
结冷胶（GG）最近被提出用于组织工程应用。GG水凝胶通过由温度变化或二价阳离子的存在诱导的物理交联方法产生。然而，物理交联方法可产生在生理条件下由于二价阳离子被单价阳离子交换而变得较弱的水凝胶。因此，这项工作提出了一类新的GG水凝胶交联的物理和化学机制。将甲基丙烯酸酯基团并入GG链中，从而产生具有高度可调的物理和机械性质的甲基丙烯酸酯化结冷胶（MeGG）水凝胶。通过核磁共振氢谱（1H-NMR）和傅里叶变换红外光谱（FTIR-ATR）对修饰后的产物进行了表征。所开发的水凝胶网络的机械性能，杨氏模量值在0.15和148千帕之间，示出通过所使用的不同交联机制来调谐。体外溶胀动力学和水解降解速率取决于用于形成水凝胶的交联机制。NIH-3 T3成纤维细胞在MeGG网络中的三维（3D）包封证明了体外生物相容性，并通过高细胞存活率得到证实。鉴于MeGG的高度可调的机械和降解特性，其可适用于广泛的组织工程方法。
Gellan Gum (GG) has been recently proposed for tissue engineering applications. GG hydrogels are produced by physical crosslinking methods induced by temperature variation or by the presence of divalent cations. However, physical crosslinking methods may yield hydrogels that become weaker in physiological conditions due to the exchange of divalent cations by monovalent ones. Hence, this work presents a new class of GG hydrogels crosslinkable by both physical and chemical mechanisms. Methacrylate groups were incorporated in the GG chain, leading to the production of a methacrylated gellan gum (MeGG) hydrogel with highly tunable physical and mechanical properties. The chemical modification was confirmed by proton nuclear magnetic resonance (1H-NMR) and Fourier transform infrared spectroscopy (FTIR-ATR). The mechanical properties of the developed hydrogel networks, with Young’s modulus values between 0.15 and 148 kPa, showed to be tuned by the different crosslinking mechanisms used. The in vitro swelling kinetics and hydrolytic degradation rate was dependent on the crosslinking mechanisms used to form the hydrogels. Three-dimensional (3D) encapsulation of NIH-3T3 fibroblast cells in MeGG networks demonstrated in vitro biocompatibility confirmed by high cell survival. Given the highly tunable mechanical and degradation properties of MeGG, it may be applicable for a wide range of tissue engineering approaches.
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影响因子：
7
作者：
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通讯作者：
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影响因子：
3.3
作者：
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影响因子：
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作者：
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通讯作者：
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