Biomimetic Liposomal Nanoplatinum for Targeted Cancer Chemophototherapy.
Biomimetic Liposomal Nanoplatinum for Targeted Cancer Chemophototherapy.
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用于靶向癌症化疗光疗的仿生脂质体纳米铂
DOI：
10.1002/advs.202003679
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Fang C
中科院分区：
文献类型：
--
作者：
Liu XL;Dong X;Yang SC;Lai X;Liu HJ;Gao Y;Feng HY;Zhu MH;Yuan Y;Lu Q;Lovell JF;Chen HZ;Fang C
光动力疗法(PDT)在肿瘤治疗中受到肿瘤缺氧的限制。铂纳米颗粒作为一种类似过氧化氢酶的纳米酶，可以通过催化供氧来增强光动力学。然而，现有的发挥纳米铂多功能抗肿瘤作用的方法并没有综合考虑纳米铂的细胞毒活性。在这里，纳米铂通过反相蒸发被加载到脂质体中。临床光敏剂维替普芬(VP)被负载到脂质双层中，以赋予PDT活性。小鼠巨噬细胞膜被杂交化到脂质体膜中以赋予仿生和靶向特征。由此产生的脂质体系统名为“纳米铂/VP@MLipo”，用于体外和体内小鼠肿瘤模型的化学光治疗。在肿瘤部位，纳米铂催化产生的氧气改善了VP介导的PDT，进而通过膜通透性触发纳米铂的释放。超小的3-5纳米纳米铂能够更好地渗透到肿瘤中，这也得益于产生的氧气，用于加强化疗。一次注射纳米铂/VP@MLipo和光照射联合化疗可抑制侵袭性4T1肿瘤的生长和肺转移，延长动物存活时间，且无明显毒性。仿生脂质体纳米铂(Nano-PT/VP@MLipo)以肿瘤部位为靶点，纳米铂催化产生的氧气增强了维替泊芬(VP)介导的光动力疗法(PDT)。PDT反过来又能渗透脂膜，有效地释放纳米铂。这些超小颗粒(3-5 nm)可以穿透更深的肿瘤组织，这也得益于产生的氧气，以加强化疗。
Photodynamic therapy (PDT) of cancer is limited by tumor hypoxia. Platinum nanoparticles (nano‐Pt) as a catalase‐like nanoenzyme can enhance PDT through catalytic oxygen supply. However, the cytotoxic activity of nano‐Pt is not comprehensively considered in the existing methods to exert their multifunctional antitumor effects. Here, nano‐Pt are loaded into liposomes via reverse phase evaporation. The clinical photosensitizer verteporfin (VP) is loaded in the lipid bilayer to confer PDT activity. Murine macrophage cell membranes are hybridized into the liposomal membrane to confer biomimetic and targeting features. The resulting liposomal system, termed “nano‐Pt/VP@MLipo,” is investigated for chemophototherapy in vitro and in vivo in mouse tumor models. At the tumor site, oxygen produced by nano‐Pt catalyzation improves the VP‐mediated PDT, which in turn triggers the release of nano‐Pt via membrane permeabilization. The ultrasmall 3–5 nm nano‐Pt enables better penetration in tumors, which is also facilitated by the generated oxygen gas, for enhanced chemotherapy. Chemophototherapy with a single injection of nano‐Pt/VP@MLipo and light irradiation inhibits the growth of aggressive 4T1 tumors and their lung metastasis, and prolongs animal survival without overt toxicity. The biomimetic liposomal nano‐Pt (nano‐Pt/VP@MLipo) targets the tumor sites, where oxygen produced by nano‐Pt catalyzation enhances the verteporfin (VP)‐mediated photodynamic therapy (PDT). PDT in turn permeabilizes the liposome membrane for efficient nano‐Pt release. These ultrasmall particles (3–5 nm) achieve penetration in deeper tumor tissue, which is also facilitated by the generated oxygen gas, for enhanced chemotherapy.
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用于靶向癌症化疗光疗的仿生脂质体纳米铂
DOI：
10.1002/advs.202003679
发表时间：
2021-04
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Liu XL;Dong X;Yang SC;Lai X;Liu HJ;Gao Y;Feng HY;Zhu MH;Yuan Y;Lu Q;Lovell JF;Chen HZ;Fang C
通讯作者：
Fang C
影响因子：
14
作者：
Luo D;Carter KA;Razi A;Geng J;Shao S;Giraldo D;Sunar U;Ortega J;Lovell JF
通讯作者：
Lovell JF
影响因子：
4.7
作者：
Ai, Xiangzhao;Hu, Ming;Xing, Bengang
通讯作者：
Xing, Bengang
影响因子：
--
作者：
Ivascu, Andrea;Kubbies, Manfred
通讯作者：
Kubbies, Manfred
纳米碗支持的脂质体改善药物装载和递送
影响因子：
10.8
作者：
Chen, Zhong-Jian;Yang, Si-Cong;Fang, Chao
通讯作者：
Fang, Chao