Responses of opioid and serotonin containing medullary raphe neurons to electroacupuncture.
Responses of opioid and serotonin containing medullary raphe neurons to electroacupuncture.
复制标题
DOI：
10.1016/j.brainres.2008.07.020
复制
发表时间：
2008-09-10
期刊：
影响因子：
2.9
通讯作者：
Longhurst, John C.
中科院分区：
文献类型：
--
作者：
Guo, Zhi-Liny;Moazzami, Ali R.;Tjen-A-Looi, Stephanie;Longhurst, John C.
延髓中线包括中缝隐核、中缝大核和中缝苍白核（NRP），参与心血管反应的调节。阿片类物质和5-羟色胺（5-HT）被认为是该区域重要的神经递质。我们以前已经证明，电针（EA）在内关，建始穴位（P5-P6，覆盖正中神经）衰减交感神经兴奋性血压反射，通过其对几个大脑区域的影响。然而，这三个中缝核在针刺反应中的作用尚不清楚。在去压力感受性神经支配和切断迷走神经的猫上，本研究评价了电针引起的中缝核c-Fos激活，并研究了其与脑啡肽和5-HT的关系。为提高脑啡肽胞体的检出率，将秋水仙碱（90-100 μg/kg）注入麻醉猫蛛网膜下腔，于针刺前28-30 h针刺P5 ~ P6穴位，电刺激或不电刺激30 min。与对照组（n=5）相比，电针组猫延髓中线3个核团的c-Fos免疫反应阳性神经元和c-Fos与脑啡肽或5-HT双标神经元的出现率均明显增高，尤以NRP（n=6，P均<0.05）为著。电针刺激后，NRP内出现c-Fos、脑啡肽和5-HT三重标记神经元。这些结果表明，延髓中缝核，特别是NRP，在EA过程中处理躯体信号，并通过阿片类或阿片能机制参与EA相关的心血管功能的调节。
The midline medulla oblongata, which includes the nucleus raphe obscurus, raphe magnus and raphe pallidus (NRP), is involved in regulation of cardiovascular responses. Opioids and serotonin (5-HT) are thought to function as important neurotransmitters in this region. We previously have demonstrated that electroacupuncture (EA) at the Neiguan-Jianshi acupoints (P5-P6, overlying the median nerves) attenuates sympathoexcitatory blood pressure reflexes through its influence on several brain regions. However, the role of these three raphe nuclei in the acupuncture responses is unknown. In baroreceptor denervated and vagotomized cats, the present study evaluated c-Fos activation in the raphe nuclei induced by EA and examined its relationship to enkephalin and 5-HT. To enhance detection of perikarya containing enkephalin, colchicine (90–100 μg/kg) was administered into the subarachnoid space in anesthetized cats 28–30 hours before the placement of acupuncture needles at P5-P6 acupoints with or without electrical stimulation for 30 min. Perikarya containing the opioid and 5-HT were found in the raphe nuclei of all animals following application of colchicine. Compared to controls without electrical stimulation (n=5), c-Fos immunoreactivity and neurons double-labeled with c-Fos and either enkephalin or 5-HT were found more frequently in all three midline medullary nuclei, especially in NRP (n=6, all P<0.05) of EA-treated cats. Moreover, neurons triple-labeled with c-Fos, enkephalin and 5-HT were noted frequently in the NRP following EA stimulation. These results suggest that the medullary raphe nuclei, particularly the NRP, process somatic signals during EA and participate in EA-related modulation of cardiovascular function through an opioid or serotonergic mechanism.
登录
查看更多内容
影响因子：
1.7
作者：
Guo, ZL;Longhurst, JC
通讯作者：
Longhurst, JC
影响因子：
2.9
作者：
Guo, ZL;Moazzami, AR;Longhurst, JC
通讯作者：
Longhurst, JC
影响因子：
2.5
作者：
Barman, SM;Gebber, GL
通讯作者：
Gebber, GL
DOI：
10.1152/ajpregu.1995.268.5.r1295
发表时间：
1995-05-01
影响因子：
2.8
作者：
COLEMAN, MJ;DAMPNEY, RAL
通讯作者：
DAMPNEY, RAL
影响因子：
2.9
作者：
Cao, WH;Morrison, SF
通讯作者：
Morrison, SF