Beclin-1 expression in normal bladder and in Cd2+ and As3+ exposed and transformed human urothelial cells (UROtsa).
Beclin-1 expression in normal bladder and in Cd2+ and As3+ exposed and transformed human urothelial cells (UROtsa).
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DOI：
10.1016/j.toxlet.2010.02.021
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发表时间：
2010-05-19
影响因子：
3.5
通讯作者：
Dunlevy, Jane R.
中科院分区：
文献类型：
--
作者：
Larson, Jennifer L.;Somji, Seema;Zhou, Xu Dong;Sens, Mary Ann;Garrett, Scott H.;Sens, Donald A.;Dunlevy, Jane R.
本研究检测了Beclin-1在正常人膀胱和镉（Cd+2）、砷（As+3）暴露及转化的尿路上皮细胞（UROtsa）中的表达。真实的时间PCR、Western分析和免疫组化结果显示，Beclin-1在正常膀胱尿路上皮细胞中有表达。还证明了亲本UROtsa细胞系以与原位尿路上皮相似的水平表达beclin-1 mRNA和蛋白。当UROtsa细胞被Cd+2或As+3恶性转化时，或者当亲本细胞急性暴露于Cd+2或As+3时，beclin-1表达水平仅发生适度的改变。虽然在单个时间点和细胞系间比较中存在显著变化，但没有证据表明与细胞系的表型特性存在剂量反应关系或相关性。对于Atg-5、Atg-7、Atg-12和LC 3B自噬相关蛋白的表达获得了类似的结果。这一发现提供了初步的证据表明，在正常膀胱的beclin-1的表达，并在beclin-1和相关蛋白的表达大的变化不会发生时，人类尿路上皮细胞恶性转化，或暴露于镉+2或As+3。
The expression of beclin-1 in normal human bladder and in Cd+2 and As+3 exposed and transformed urothelial cells (UROtsa) was examined in this study. It was shown using a combination of real time PCR, western analysis and immunohistochemistry that beclin-1 was expressed in the urothelial cells of the normal bladder. It was also demonstrated that the parental UROtsa cell line expressed beclin-1 mRNA and protein at levels similar to that of the in situ urothelium. The level of beclin-1 expression underwent only modest alterations when the UROtsa cells were malignantly transformed by Cd+2 or As+3 or when the parental cells were exposed acutely to Cd+2 or As+3. While there were instances of significant alterations at individual time points and within cell line-to-cell line comparisons there was no evidence of a dose response relationship or correlations to the phenotypic properties of the cell lines. Similar results were obtained for the expression of the Atg-5, Atg-7, Atg-12 and LC3B autophagy-related proteins. The findings provide initial evidence for beclin-1 expression in normal bladder and that large alterations in the expression of beclin-1 and associated proteins do not occur when human urothelial cells are malignantly transformed with, or exposed to, either Cd+2 or As+3.
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影响因子：
10.4
作者：
Garrett SH;Somji S;Todd JH;Sens DA
通讯作者：
Sens DA
影响因子：
3.5
作者：
Chai, Chee-Yin;Huang, Ya-Chun;Chen, Wan-Tzu
通讯作者：
Chen, Wan-Tzu
影响因子：
--
作者：
Petzoldt, JL;Leigh, IM;Masters, JRW
通讯作者：
Masters, JRW
影响因子：
4.1
作者：
Somji, Seema;Zhou, Xu Dong;Sens, Donald. A.
通讯作者：
Sens, Donald. A.
影响因子：
8
作者：
Sinha, S.;Levine, B.
通讯作者：
Levine, B.