In Vivo Imaging Reveals Existence of Crypt Fission and Fusion in Adult Mouse Intestine.
In Vivo Imaging Reveals Existence of Crypt Fission and Fusion in Adult Mouse Intestine.
复制标题
体内成像显示成年小鼠肠道中存在隐窝分裂和融合现象
DOI：
10.1053/j.gastro.2017.05.019
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发表时间：
2017-09
期刊：
影响因子：
29.4
通讯作者：
Snippert HJ
中科院分区：
文献类型：
--
作者：
Bruens L;Ellenbroek SIJ;van Rheenen J;Snippert HJ
肠上皮是一个重复的隐窝和绒毛单位与干细胞在底部的隐窝。在出生后的发育过程中，隐窝通过分裂繁殖，从1个父母隐窝产生2个女儿隐窝。在成年人的肠道，隐窝分裂观察到在一个低频率。在Lgr 5EGFP-Ires-CreERT 2小鼠中使用活体显微镜，我们用单细胞分辨率监测了多天的个体隐窝动态。我们发现了一种几乎完全相反的细胞分裂现象，即2个细胞融合成1个子细胞。对4只小鼠的819个隐窝进行了检查，发现3.5% ± 0.6%的隐窝处于分裂过程中，而4.1% ± 0.9%的隐窝处于融合过程中。在小鼠的一生中，隐窝分裂和融合作为一种抵消过程，可以调节隐窝的数量。确定调节隐窝分裂和融合速率的机制可以为肠道适应改变的环境条件和疾病发病机制提供见解。
The intestinal epithelium is a repetitive sheet of crypt and villus units with stem cells at the bottom of the crypts. During postnatal development, crypts multiply via fission, generating 2 daughter crypts from 1 parental crypt. In the adult intestine, crypt fission is observed at a low frequency. Using intravital microscopy in Lgr5EGFP-Ires-CreERT2 mice, we monitored individual crypt dynamics over multiple days with single-cell resolution. We discovered the existence of crypt fusion, an almost exact reverse phenomenon of crypt fission, in which 2 crypts fuse into 1 daughter crypt. Examining 819 crypts in 4 mice, we found that 3.5% ± 0.6% of all crypts were in the process of fission, whereas 4.1 ± 0.9% of all crypts were undergoing crypt fusion. As counteracting processes, crypt fission and fusion could regulate crypt numbers during the lifetime of a mouse. Identifying the mechanisms that regulate rates of crypt fission and fusion could provide insights into intestinal adaptation to altered environmental conditions and disease pathogenesis.
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DOI：
10.1073/pnas.0505903103
发表时间：
2006-01-17
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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DOI：
10.1111/j.1365-2184.1975.tb01219.x
发表时间：
1975-01-01
期刊：
CELL AND TISSUE KINETICS
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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