Down-regulated GABAergic expression in the olfactory bulb layers of the mouse deficient in monoamine oxidase B and administered with amphetamine.
Down-regulated GABAergic expression in the olfactory bulb layers of the mouse deficient in monoamine oxidase B and administered with amphetamine.
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DOI：
10.1007/s10571-009-9475-2
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发表时间：
2010-05
影响因子：
4
通讯作者：
Tien, Tai-Wei
中科院分区：
文献类型：
--
作者：
Yin, Hsiang-Shu;Chen, Kevin;Shih, Jean C.;Tien, Tai-Wei
本研究主要探讨单胺氧化酶B （MAOB）活性在调节连接边缘系统的主嗅球（OlfB）不同层中谷氨酸脱羧酶67 （GAD67）表达中的作用。此外，在缺乏MAOB活性的情况下，GAD67对安非他命扰动的反应进行了研究。对成年野生型（WT）和MAOB基因敲除（KO）小鼠反复腹腔注射生理盐水或安非他明（5 mg/kg）（2剂量/天，共7剂量）制备的OlfB切片进行免疫细胞化学分析。经盐水处理的KO小鼠肾小球（GloL）和二尖瓣细胞层（ML）的GAD67免疫反应性水平比WT低约25%和38%，而KO和WT的外丛状细胞层或颗粒细胞层（GraL）的GAD67免疫反应性水平相似。在GloL中，KO小鼠的酪氨酸羟化酶水平比WT低39%。除ML外，安非他明暴露使WT层中的GAD67水平下调46%至52%，KO层中的GAD67水平下调65%至71%。GraL中的GAD67水平可能通过激活CREB来调节，因为磷酸化的CREB与GAD67共存，并且安非他明暴露降低了表达GAD67的pCREB神经元的百分比。这些数据表明，MAOB的活性可以调节GAD67和pCREB的正常表达和安非他明干扰的表达。因此，OlfB的MAOB活性、GABA含量与嗅觉之间存在相互作用。
This study explores primarily the role of the activity of monoamine oxidase B (MAOB) in the regulation of glutamic acid decarboxylase67 (GAD67) expression in distinct layers of main olfactory bulb (OlfB), which links the limbic system. Moreover, the response of GAD67 was investigated to amphetamine perturbation in the absence of MAOB activity. Immunocytochemical analysis was performed on OlfB sections prepared from the adult wild type (WT) and the MAOB gene-knockout (KO) mice after receiving repeated intraperitoneal injections (2 doses/day, total 7 doses) of saline or amphetamine, 5 mg/kg. The levels of the GAD67 immunoreactivity were approximate 25% and 38% lower in respective glomerular (GloL) and mitral cell layers (ML) of saline-treated KO mice than that of WT, whereas similar in the external plexiform or granule cell layers (GraL) of the KO and WT. In the GloL, the level of tyrosine hydroxylase was 39% lower in the KO mice than WT, implicating different dopamine content in the KO from WT. The amphetamine exposure down-regulated the levels of GAD67 in the WT layers by 46% to 52%, and in KO layers 65% to 71%, except ML. The GraL GAD67 level may be regulated by the activation of CREB, as the phosphorylated (p) CREB coexisted with GAD67, and the percentage of GAD67-expressing pCREB neurons was decreased by the amphetamine exposure. The data indicate that the activity of MAOB could modulate the regular and amphetamine-perturbed expression of GAD67 and pCREB. Thus, interactions are suggested among the MAOB activity, GABA content of OlfB and olfaction.
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影响因子：
2.8
作者：
Coronas, V;Srivastava, LK;Moyse, E
通讯作者：
Moyse, E
影响因子：
5.8
作者：
Parent, MB;Habib, MK;Baker, GB
通讯作者：
Baker, GB
DOI：
10.1073/pnas.79.20.6385
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LEVITT, P;PINTAR, JE;BREAKEFIELD, XO
通讯作者：
BREAKEFIELD, XO
影响因子：
3.6
作者：
Federici, M;Geracitano, R;Mercuri, NB
通讯作者：
Mercuri, NB
影响因子：
2.9
作者：
Karler, R;Bedingfield, JB;Calder, LD
通讯作者：
Calder, LD