Tracing the lactate shuttle to the mitochondrial reticulum.
Tracing the lactate shuttle to the mitochondrial reticulum.
复制标题
追踪乳酸穿梭至线粒体网。
DOI：
10.1038/s12276-022-00802-3
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发表时间：
2022-09
影响因子：
12.8
通讯作者：
Arevalo, Jose A.
中科院分区：
文献类型：
--
作者：
Brooks, George A.;Curl, Casey C.;Leija, Robert G.;Osmond, Adam D.;Duong, Justin J.;Arevalo, Jose A.
采用乳酸盐、葡萄糖、甘油和脂肪酸同位素示踪剂的同位素示踪剂输注研究是在全身水平推导和证明乳酸穿梭的核心。与进行组织代谢物浓度测量的能力以及通过动脉-静脉差（a-v）和组织床（包括骨骼肌、心脏和脑）上的血流测量来确定单向和净代谢物交换的能力相一致，乳酸盐在器官和组织内穿梭变得明显。从对男性和女性的广泛研究中，休息或锻炼，耐力训练之前或之后，在海平面或高海拔，我们现在知道器官-器官，细胞-细胞和细胞内乳酸穿梭机连续运行。通过乳酸穿梭，燃料-能量底物可以在生产者（驱动者）细胞（如骨骼肌中的那些）和消耗者（受体）细胞（如脑、心脏、肌肉、肝脏和肾脏中的那些）之间交换。在组织内，乳酸可以在肌肉床内的白色和红色纤维之间以及脑中的星形胶质细胞和神经元之间交换。在细胞内，乳酸可以在胞质溶胶和线粒体之间以及胞质溶胶和过氧化物酶体之间交换。乳酸盐在驱动细胞和受体细胞之间穿梭取决于受体细胞中线粒体呼吸器产生的浓度梯度，用于乳酸盐的氧化处理。使用同位素示踪技术的研究显着提高了对乳酸盐（一种代谢物）如何在正常代谢过程中作为燃料产生以及响应运动、在全身移动或“穿梭”的了解。美国加州大学伯克利分校的乔治布鲁克斯及其同事回顾了对乳酸穿梭的理解历史，这在很大程度上是由在休息和运动期间使用同位素示踪剂输注的人体研究所提供的。这些研究强调了连续的器官-器官、细胞-细胞和细胞内乳酸穿梭。乳酸盐在骨骼肌细胞等生产细胞和心脏、大脑等组织中的消耗细胞之间移动，在这些组织中，乳酸盐作为能量来源比葡萄糖更受欢迎。穿梭依赖于受体细胞中线粒体网络产生的乳酸浓度梯度。乳酸盐通过氧化处理。
Isotope tracer infusion studies employing lactate, glucose, glycerol, and fatty acid isotope tracers were central to the deduction and demonstration of the Lactate Shuttle at the whole-body level. In concert with the ability to perform tissue metabolite concentration measurements, as well as determinations of unidirectional and net metabolite exchanges by means of arterial–venous difference (a-v) and blood flow measurements across tissue beds including skeletal muscle, the heart and the brain, lactate shuttling within organs and tissues was made evident. From an extensive body of work on men and women, resting or exercising, before or after endurance training, at sea level or high altitude, we now know that Organ–Organ, Cell–Cell, and Intracellular Lactate Shuttles operate continuously. By means of lactate shuttling, fuel-energy substrates can be exchanged between producer (driver) cells, such as those in skeletal muscle, and consumer (recipient) cells, such as those in the brain, heart, muscle, liver and kidneys. Within tissues, lactate can be exchanged between white and red fibers within a muscle bed and between astrocytes and neurons in the brain. Within cells, lactate can be exchanged between the cytosol and mitochondria and between the cytosol and peroxisomes. Lactate shuttling between driver and recipient cells depends on concentration gradients created by the mitochondrial respiratory apparatus in recipient cells for oxidative disposal of lactate. Studies using isotope tracer technologies have significantly improved understanding of how lactate, a metabolite produced as fuel during normal metabolism and in response to exercise, moves or ‘shuttles’ throughout the body. George Brooks and colleagues at the University of California, Berkeley, USA, reviewed the history of the understanding of lactate shuttling, which has largely been informed by human studies using isotope tracer infusions during rest and exercise. Such research highlights continuous organ–organ, cell–cell, and intracellular lactate shuttling. Lactate moves between producer cells such as skeletal muscle cells and consumer cells in tissues including the heart and brain, where it is preferred over glucose as an energy source. Shuttling depends on lactate concentration gradients created by mitochondrial networks in recipient cells. Lactate is disposed of via oxidation.
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影响因子：
7.8
作者：
Baba, N;Sharma, H M
通讯作者：
Sharma, H M
DOI：
10.1111/j.1748-1716.1936.tb00435.x
发表时间：
1936-01-01
期刊：
SKANDINAVISCHES ARCHIV FUR PHYSIOLOGIE
影响因子：
--
作者：
Bang, O
通讯作者：
Bang, O
影响因子：
3.3
作者：
BERTOCCI, LA;HALLER, RG;LEWIS, SF
通讯作者：
LEWIS, SF
DOI：
10.1152/ajpendo.1999.277.1.e81
发表时间：
1999-07-01
影响因子：
5.1
作者：
Bergman, BC;Butterfield, GE;Brooks, GA
通讯作者：
Brooks, GA
DOI：
10.1152/ajpendo.1999.276.1.e106
发表时间：
1999-01-01
影响因子：
5.1
作者：
Bergman, BC;Butterfield, GE;Brooks, GA
通讯作者：
Brooks, GA