Automatic cortical thickness analysis on rodent brain.
Automatic cortical thickness analysis on rodent brain.
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DOI：
10.1117/12.878305
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发表时间：
2011-03-15
期刊：
影响因子：
--
通讯作者：
Oguz I
中科院分区：
文献类型：
--
作者：
Lee J;Ehlers C;Crews F;Niethammer M;Budin F;Paniagua B;Sulik K;Johns J;Styner M;Oguz I
大脑皮质的局部差异是人类和动物大脑研究中最有用的形态计量学特征之一。已经开发了许多工具和方法来自动测量和分析人脑的皮质厚度。然而，由于规模不同，这些工具不能直接应用于啮齿动物的大脑；即使是成年啮齿动物的大脑也比人类的大脑小50到100倍。本文描述了一种自动测量小鼠和大鼠大脑皮质厚度的算法。该算法包括三个步骤：分割、厚度测量和实验组间的统计分析。分割步骤提供新大脑皮层与其他大脑结构的分离，因此是厚度测量的预处理步骤。在厚度测量步骤中，通过求解拉普拉斯偏微分方程和输运方程来计算厚度。拉普拉斯偏微分方程组首先产生流线，作为皮质柱的类比；传输方程计算流线的长度。结果存储为新大脑皮层曲面上的厚度贴图。对于统计分析，重要的是相应点处的样本厚度。这是通过粒子对应算法实现的，该算法最小化动态移动的样本点之间的熵，称为粒子。由于对应算法的计算代价可能会限制对应点的数目，因此我们采用基于薄板样条的插值法来增加对应样本点的数目。作为一个驱动应用，我们测量了厚度差异，以评估持续到成年的青少年间歇性酒精暴露的影响，并在对照组和暴露组之间进行了t检验。我们在两个半球发现了明显不同的区域。
Localized difference in the cortex is one of the most useful morphometric traits in human and animal brain studies. There are many tools and methods already developed to automatically measure and analyze cortical thickness for the human brain. However, these tools cannot be directly applied to rodent brains due to the different scales; even adult rodent brains are 50 to 100 times smaller than humans. This paper describes an algorithm for automatically measuring the cortical thickness of mouse and rat brains. The algorithm consists of three steps: segmentation, thickness measurement, and statistical analysis among experimental groups. The segmentation step provides the neocortex separation from other brain structures and thus is a preprocessing step for the thickness measurement. In the thickness measurement step, the thickness is computed by solving a Laplacian PDE and a transport equation. The Laplacian PDE first creates streamlines as an analogy of cortical columns; the transport equation computes the length of the streamlines. The result is stored as a thickness map over the neocortex surface. For the statistical analysis, it is important to sample thickness at corresponding points. This is achieved by the particle correspondence algorithm which minimizes entropy between dynamically moving sample points called particles. Since the computational cost of the correspondence algorithm may limit the number of corresponding points, we use thin-plate spline based interpolation to increase the number of corresponding sample points. As a driving application, we measured the thickness difference to assess the effects of adolescent intermittent ethanol exposure that persist into adulthood and performed t-test between the control and exposed rat groups. We found significantly differing regions in both hemispheres.
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DOI：
10.1007/978-3-642-02498-6_54
发表时间：
2009
期刊：
LECTURE NOTES IN ARTIFICIAL INTELLIGENCE
影响因子：
--
作者：
Oguz, Ipek;Niethammer, Marc;Cates, Josh;Whitaker, Ross;Fletcher, Thomas;Vachet, Clement;Styner, Martin
通讯作者：
Styner, Martin
影响因子：
5.7
作者：
Kim, JS;Singh, V;Evans, AC
通讯作者：
Evans, AC
影响因子：
5.7
作者：
Lerch, Jason P.;Carroll, Jeffrey B.;Henkelman, R. Mark
通讯作者：
Henkelman, R. Mark
影响因子：
3.7
作者：
Salat, DH;Buckner, RL;Fischl, B
通讯作者：
Fischl, B
影响因子：
3.7
作者：
He, Yong;Chen, Zhang J.;Evans, Alan C.
通讯作者：
Evans, Alan C.