The effects of different enrofloxacin dosages on clinical efficacy and resistance development in chickens experimentally infected with Salmonella Typhimurium.
The effects of different enrofloxacin dosages on clinical efficacy and resistance development in chickens experimentally infected with Salmonella Typhimurium.
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不同剂量恩诺沙星对鼠伤寒沙门氏菌实验感染鸡临床疗效及耐药性产生的影响
DOI：
10.1038/s41598-017-12294-7
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发表时间：
2017-09-15
影响因子：
4.6
通讯作者：
Yuan Z
中科院分区：
文献类型：
--
作者：
Li J;Hao H;Cheng G;Wang X;Ahmed S;Shabbir MAB;Liu Z;Dai M;Yuan Z
为探讨提高临床疗效和减少耐药性的最佳剂量，建立了恩诺沙星的药动学/药效学模型。本试验研究了恩诺沙星对沙门氏菌实验感染鸡的清除效果及对沙门氏菌和大肠菌群的抗性选择。和对照组。治疗持续时间为三轮7天治疗，与7天停药交替。结果表明，100 mg/kg b.w.第二轮停药期结束至试验结束（第28-42天），恩诺沙星完全杀灭沙门氏菌，但仍有4.0-60.8%的大肠菌群产生耐药。基于PK/PD的剂量（4 mg/kg b.w.）在第一次治疗期间有效地减少了沙门氏菌。然而，停止用药后，沙门氏菌在鸡群中繁殖并持续到最后，敏感性降低（MICCIP = 0.03-0.25 mg/L）。选择低频率（5-9.5%）的耐药大肠菌群（第39-42天）。恩诺沙星剂量为0.1 mg/kg b.w.不能消除沙门氏菌和选定的大肠菌群，敏感性略有下降（MICENR = 0.25 mg/L）。总之，高剂量恩诺沙星（100 mg/kg b.w.）可以有效治疗沙门氏菌感染，同时最大限度地减少沙门氏菌和大肠菌群的抗性选择。
To investigate the optimal dosage which can improve clinical efficacy and minimize resistance, pharmacokinetics/pharmacodynamics model of enrofloxacin was established. Effect of enrofloxacin treatments on clearance ofSalmonellain experimentally infected chickens and simultaneously resistance selection inSalmonellaand coliforms were evaluated in three treatment groups (100, PK/PD designed dosage of 4, 0.1 mg/kg b.w.) and a control group. Treatment duration was three rounds of 7-day treatment alternated with 7-day withdrawal. Results showed that 100 mg/kg b.w. of enrofloxacin completely eradicatedSalmonella, but resistant coliforms (4.0–60.8%) were selected from the end of the second round’s withdrawal period till the end of the experiment (days 28–42). PK/PD based dosage (4 mg/kg b.w.) effectively reducedSalmonellafor the first treatment duration. However upon cessation of medication,Salmonellarepopulated chickens and persisted till the end with reduced susceptibility (MICCIP = 0.03–0.25 mg/L). Low frequency (5–9.5%) of resistant coliforms was selected (days 39–42). Enrofloxacin at dosage of 0.1 mg/kg b.w. was not able to eliminateSalmonellaand selected coliforms with slight decreased susceptibility (MICENR = 0.25 mg/L). In conclusion, short time treatment (7 days) of enrofloxacin at high dosage (100 mg/kg b.w.) could be effective in treatingSalmonellainfection while minimizing resistance selection in bothSalmonellaand coliforms.
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影响因子：
3.3
作者：
Hoffmann M;Zhao S;Pettengill J;Luo Y;Monday SR;Abbott J;Ayers SL;Cinar HN;Muruvanda T;Li C;Allard MW;Whichard J;Meng J;Brown EW;McDermott PF
通讯作者：
McDermott PF
影响因子：
4.9
作者：
Giraud, E;Brisabois, A;Chaslus-Dancla, E
通讯作者：
Chaslus-Dancla, E
影响因子：
5.2
作者：
Akiyama, Tatsuya;Khan, Ashraf A.
通讯作者：
Khan, Ashraf A.
影响因子：
2.4
作者：
Anadón, A;Martínez-Larrañaga, MR;Martínez, M
通讯作者：
Martínez, M
影响因子：
3
作者：
Giraud, E;Cloeckaert, A;Chaslus-Dancla, E
通讯作者：
Chaslus-Dancla, E