The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model.
The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model.
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DOI：
10.1186/s40729-017-0112-4
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发表时间：
2018-01-13
影响因子：
2.7
通讯作者：
Koyano K
中科院分区：
文献类型：
--
作者：
Kanazawa M;Atsuta I;Ayukawa Y;Yamaza T;Kondo R;Matsuura Y;Koyano K
多能间充质干细胞（MSC）在临床上用于再生医学。我们之前的报告显示，系统注射 MSC 可以改善种植体周围的密封并加速组织愈合。然而，必须考虑全身性 MSC 给药的风险，包括肺栓塞；因此，必须评估其本地应用的临床安全性和有效性。我们使用大鼠口腔植入模型研究了局部和全身 MSC 应用之间治疗效果的差异。分离并培养扩增大鼠骨髓来源的 MSC。拔除大鼠右上颌第一磨牙，并用实验性钛种植体替换。 24小时后，将MSCs（1 × 106/ml）分别通过尾静脉（全身组）或颊皮下组织（局部组）全身或局部注射到受体大鼠体内。在不存在 MSC 的情况下治疗的大鼠被纳入作为对照（对照组）。 4 周后对上颌上皮进行组织学评估，以评估层粘连蛋白 332 (Ln-332) 分布和辣根过氧化物酶侵入，分别作为种植体周围上皮 (PIE) 形成和 PIE 密封种植体表面的指标。通过共培养测定 MSC 对大鼠口腔上皮细胞 (OEC) 形态的影响。系统性组 MSC 早期在种植体周围粘膜处积累，而局部组 MSC 在各个器官中观察到，随后在种植体表面周围积累。与局部组和对照组相比，全身组的种植体界面处的 PIE 形成和 Ln-332 阳性染色增强。此外，与低密度 MSC 共培养相比，高密度下 OEC 在植入物上的粘附性降低。在增强钛种植体周围 PIE 密封方面，局部 MSC 注射比全身 MSC 注射更无效。因此，尽管局部 MSC 给药具有广泛的应用，但在临床使用之前还需要进一步研究以了解这种方法的确切细胞和分子机制。
Multipotent mesenchymal stem cells (MSCs) are used clinically in regenerative medicine. Our previous report showed systemically injected MSCs improved peri-implant sealing and accelerated tissue healing. However, the risks of systemic MSC administration, including lung embolism, must be considered; therefore, their local application must be assessed for clinical safety and efficacy. We investigated differences in treatment effect between local and systemic MSC application using a rat oral implantation model. Rat bone marrow-derived MSCs were isolated and culture-expanded. The rat’s right maxillary first molars were extracted and replaced with experimental titanium implants. After 24 h, MSCs (1 × 106/ml) were systemically or locally injected into recipient rats via the tail vein (systemic group) or buccal subcutaneous tissue (local group), respectively. Rats treated in the absence of MSCs were included as a control (control group). The maxillary epithelium was assessed histologically after 4 weeks to evaluate laminin-332 (Ln-332) distribution and horseradish peroxidase invasion, as indicators of peri-implant epithelium (PIE) formation and PIE sealing to the implant surface, respectively. The effect of MSCs on rat oral epithelial cell (OEC) morphology was determined by coculture. Systemic group MSCs accumulated early at the peri-implant mucosa, while local group MSCs were observed in various organs prior to later accumulation around the implant surface. PIE formation and Ln-332-positive staining at the implant interface were enhanced in the systemic group compared with the local and control groups. Furthermore, OEC adherence on implants was reduced in high-density compared with low-density MSC cocultures. Local MSC injection was more ineffective than systemic MSC injection at enhancing PIE sealing around titanium implants. Thus, although local MSC administration has a wide range of applications, further investigations are needed to understand the exact cellular and molecular mechanisms of this approach prior to clinical use.
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影响因子：
3.7
作者：
Kondo R;Atsuta I;Ayukawa Y;Yamaza T;Matsuura Y;Furuhashi A;Tsukiyama Y;Koyano K
通讯作者：
Koyano K
影响因子：
3.3
作者：
Park, Won Soon;Sung, Se In;Chang, Yun Sil
通讯作者：
Chang, Yun Sil
DOI：
10.1083/jcb.148.2.253
发表时间：
2000-01-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
O'Connor KL;Nguyen BK;Mercurio AM
通讯作者：
Mercurio AM
影响因子：
14
作者：
Atsuta, I;Yamaza, T;Tanaka, T
通讯作者：
Tanaka, T
影响因子：
7.5
作者：
Bazhanov N;Ylostalo JH;Bartosh TJ;Tiblow A;Mohammadipoor A;Foskett A;Prockop DJ
通讯作者：
Prockop DJ