A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing
A Silent Exonic Mutation in a Rice Integrin-α FG-GAP Repeat-Containing Gene Causes Male-Sterility by Affecting mRNA Splicing
复制标题
水稻整合素-α FG-GAP 重复基因中的沉默外显子突变通过影响 mRNA 剪接导致雄性不育
DOI：
10.3390/ijms21062018
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发表时间：
2020-03
期刊：
影响因子：
--
通讯作者：
Li Shuangcheng
中科院分区：
文献类型：
--
作者：
Zou Ting;Zhou Dan;Li Wenjie;Yuan Guoqiang;Tao Yang;He Zhiyuan;Zhang Xu;Deng Qiming;Wang Shiquan;Zheng Aiping;Zhu Jun;Liang Yueyang;Liu Huainian;Wang Aijun;Wang Lingxia;Li Ping;Li Shuangcheng
Pollen development plays crucial roles in the life cycle of higher plants. Here we characterized a rice mutant with complete male-sterile phenotype, pollen-less 1 (pl1). pl1 exhibited smaller anthers with arrested pollen development, absent Ubisch bodies, necrosis-like tapetal hypertrophy, and smooth anther cuticular surface. Molecular mapping revealed a synonymous mutation in the fourth exon of PL1 co-segregated with the mutant phenotype. This mutation disrupts the exon-intron splice junction in PL1, generating aberrant mRNA species and truncated proteins. PL1 is highly expressed in the tapetal cells of developing anther, and its protein is co-localized with plasma membrane (PM) and endoplasmic reticulum (ER) signal. PL1 encodes an integrin-α FG-GAP repeat-containing protein, which has seven β-sheets and putative Ca2+-binding motifs and is broadly conserved in terrestrial plants. Our findings therefore provide insights into both the role of integrin-α FG-GAP repeat-containing protein in rice male fertility and the influence of exonic mutation on intronic splice donor site selection.
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