Morphogenesis and cell ordering in confined bacterial biofilms
Morphogenesis and cell ordering in confined bacterial biofilms
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受限细菌生物膜中的形态发生和细胞排序
DOI：
10.1073/pnas.2107107118
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yan, Jing
中科院分区：
文献类型：
--
作者：
Zhang, Qiuting;Li, Jian;Nijjer, Japinder;Lu, Haoran;Kothari, Mrityunjay;Alert, Ricard;Cohen, Tal;Yan, Jing
生物膜是由细胞外基质包围的细菌细胞聚集体。在固体基质上生物膜生长的研究已经取得了很大的进展，然而，很少有人知道生物膜发展的生物物理机制在三维密闭环境中，生物膜居住的细胞必须推动，甚至破坏周围的环境增殖。在这里，结合单细胞成像，诱变和流变学测量，我们揭示了嵌入水凝胶的霍乱弧菌生物膜的关键形态发生步骤，因为它们从初始大小增长了四个数量级。我们发现，嵌入式生物膜的形态动力学和细胞排序是从根本上不同于那些在平坦表面上的生物膜。处理嵌入式生物膜作为夹杂物生长在弹性介质中，我们定量地表明，生物膜和它的环境之间的刚度对比决定生物膜的形态和内部结构，选择之间的球形生物膜没有细胞排序和扁圆形椭球体生物膜高细胞排序。当嵌入到硬凝胶中时，细胞自组织成双极结构，类似于双极性液晶滴中的分子排序。体外生物力学分析表明，细胞有序化是由特定基质成分介导的跨生物膜-环境界面的应力传递引起的。我们的成像技术和理论方法可推广到其他生物膜形成物种，并可能在感染期间嵌入粘液或宿主组织中的生物膜。我们的研究结果开辟了一条途径，以了解有限的细胞群落如何通过其固有的发育程序和环境所施加的机械约束之间的妥协来生长。
Biofilms are aggregates of bacterial cells surrounded by an extracellular matrix. Much progress has been made in studying biofilm growth on solid substrates; however, little is known about the biophysical mechanisms underlying biofilm development in three-dimensional confined environments in which the biofilm-dwelling cells must push against and even damage the surrounding environment to proliferate. Here, combining single-cell imaging, mutagenesis, and rheological measurement, we reveal the key morphogenesis steps ofVibrio choleraebiofilms embedded in hydrogels as they grow by four orders of magnitude from their initial size. We show that the morphodynamics and cell ordering in embedded biofilms are fundamentally different from those of biofilms on flat surfaces. Treating embedded biofilms as inclusions growing in an elastic medium, we quantitatively show that the stiffness contrast between the biofilm and its environment determines biofilm morphology and internal architecture, selecting between spherical biofilms with no cell ordering and oblate ellipsoidal biofilms with high cell ordering. When embedded in stiff gels, cells self-organize into a bipolar structure that resembles the molecular ordering in nematic liquid crystal droplets. In vitro biomechanical analysis shows that cell ordering arises from stress transmission across the biofilm–environment interface, mediated by specific matrix components. Our imaging technique and theoretical approach are generalizable to other biofilm-forming species and potentially to biofilms embedded in mucus or host tissues as during infection. Our results open an avenue to understand how confined cell communities grow by means of a compromise between their inherent developmental program and the mechanical constraints imposed by the environment.
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生长中的细菌菌落从单层到多层的转变。
影响因子：
8.6
作者：
Zhihong You;D. Pearce;A. Sengupta;L. Giomi
通讯作者：
L. Giomi
DOI：
10.1126/science.aaz4547
发表时间：
2020-03-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Duclos G;Adkins R;Banerjee D;Peterson MSE;Varghese M;Kolvin I;Baskaran A;Pelcovits RA;Powers TR;Baskaran A;Toschi F;Hagan MF;Streichan SJ;Vitelli V;Beller DA;Dogic Z
通讯作者：
Dogic Z
影响因子：
19.6
作者：
Copenhagen, Katherine;Alert, Ricard;Shaevitz, Joshua W.
通讯作者：
Shaevitz, Joshua W.
生长的细菌生物膜中流动引起的对称性破坏
DOI：
10.1101/627208
发表时间：
2019
期刊：
bioRxiv
影响因子：
--
作者：
P. Pearce;Boya Song;Dominic J. Skinner;Rachel Mok;Raimo Hartmann;Praveen K. Singh;H. Jeckel;J. Oishi;K. Drescher;J. Dunkel
通讯作者：
J. Dunkel
非线性包含理论在约束体的生长和形态发生中的应用
影响因子：
5.3
作者：
Li, Jian;Kothari, Mrityunjay;Chockalingam, S.;Henzel, Thomas;Zhang, Qiuting;Li, Xuanhe;Yan, Jing;Cohen, Tal
通讯作者：
Cohen, Tal