Cytoskyrins and cytosporones produced by Cytospora sp. CR200: taxonomy, fermentation and biological activities.
Cytoskyrins and cytosporones produced by Cytospora sp. CR200: taxonomy, fermentation and biological activities.
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DOI：
10.3390/md503071
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发表时间：
2007-07-19
期刊：
影响因子：
5.4
通讯作者：
Brady SF
中科院分区：
文献类型：
--
作者：
Singh MP;Janso JE;Brady SF
In screening endophytic fungi from Costa Rica for bioactivity, fungal culture CR200, isolated from a buttonwood tree, was found to contain compounds that initiate DNA damage in a test strain of E. coli (Biochemical Induction Assay, BIA) and inhibit growth of Gram-positive bacteria, including antibiotic-resistant strains. Two new bisanthraquinones (cytoskyrins A and B) and five new related octaketides (cytosporones A-E) were isolated from fermentation broths of this fungus. Cytoskyrin A exhibited potent in-vitro antibacterial (MICs against Gram-positive bacteria, 0.03 – 0.25 μg/mL) and DNA-damaging activities (10 ng/spot), whereas cytoskyrin B was inactive in these assays. Among the cytosporones, only D and E exhibited Gram-positive activity, but they were inactive in the BIA. Mechanistically, cytoskyrin A specifically inhibited DNA synthesis in E. coli imp at its MIC; however, it also moderately inhibited protein synthesis at 2x its MIC. Cytoskyrin A exhibited poor cytotoxicity against tumor cell lines (IC50 > 5 μg/mL) compared to known antitumor agents. The nuclear ribosomal internal transcribed spacer region of CR200 was found to share highest similarity (94–96%) with Cytospora spp. Micro- and macroscopic morphological observations of the conidia and conidiomata, respectively, also suggested this fungus to be a Cytospora sp.
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影响因子：
3.6
作者：
He, HY;Janso, JE;Carter, GT
通讯作者：
Carter, GT
影响因子：
2.5
作者：
KUBOTA, T;TAKAHARA, T;KITAJIMA, M
通讯作者：
KITAJIMA, M
影响因子：
4.9
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Singh, MP;Petersen, PJ;Greenstein, M
通讯作者：
Greenstein, M
影响因子：
5.2
作者：
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通讯作者：
Clardy, J
影响因子：
1.7
作者：
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通讯作者：
Shibuya, H