Reduced satellite cell numbers and myogenic capacity in aging can be alleviated by endurance exercise.
Reduced satellite cell numbers and myogenic capacity in aging can be alleviated by endurance exercise.
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DOI：
10.1371/journal.pone.0013307
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发表时间：
2010-10-12
期刊：
影响因子：
3.7
通讯作者：
Benayahu D
中科院分区：
文献类型：
--
作者：
Shefer G;Rauner G;Yablonka-Reuveni Z;Benayahu D
肌肉再生依赖于卫星细胞，即位于肌纤维表面的肌源性干细胞。这些细胞的数量减少和/或生肌能力降低可能会阻碍适当的维护，并导致与年龄相关的肌肉质量和修复能力下降。耐力运动被证明可以改善肌肉性能;然而，对衰老中卫星细胞的直接影响尚未完全确定。在这里，我们专注于描述中等强度耐力运动对卫星细胞的影响，作为减轻衰老不利影响的可能手段。年轻和老年大鼠的两种性别进行了13周的跑步机运行或保持久坐不动。腓肠肌的影响，年龄，性别和运动卫星细胞数量和生肌能力进行了评估。基于Pax 7免疫染色在新鲜分离的肌纤维中鉴定卫星细胞（即，离体）。在克隆测定（体外）中测定单个肌纤维相关细胞产生肌源性后代的能力。我们发现，与年龄相关的卫星细胞数量减少，并在老年久坐不动的大鼠肌源性克隆的百分比。运动后，年轻和老年大鼠中含有更多卫星细胞的肌纤维增加，来自老年大鼠的肌源性克隆的百分比增加。老年大鼠卫星细胞水平的变化伴随着对瘦-脂肪胃肌组成和自发运动水平的积极影响。这些数据的重要性在于，它们表明耐力运动介导的卫星数量和生肌特性的增加可能改善衰老中的肌纤维维持。
Muscle regeneration depends on satellite cells, myogenic stem cells that reside on the myofiber surface. Reduced numbers and/or decreased myogenic aptitude of these cells may impede proper maintenance and contribute to the age-associated decline in muscle mass and repair capacity. Endurance exercise was shown to improve muscle performance; however, the direct impact on satellite cells in aging was not yet thoroughly determined. Here, we focused on characterizing the effect of moderate-intensity endurance exercise on satellite cell, as possible means to attenuate adverse effects of aging. Young and old rats of both genders underwent 13 weeks of treadmill-running or remained sedentary. Gastrocnemius muscles were assessed for the effect of age, gender and exercise on satellite-cell numbers and myogenic capacity. Satellite cells were identified in freshly isolated myofibers based on Pax7 immunostaining (i.e., ex-vivo). The capacity of individual myofiber-associated cells to produce myogenic progeny was determined in clonal assays (in-vitro). We show an age-associated decrease in satellite-cell numbers and in the percent of myogenic clones in old sedentary rats. Upon exercise, there was an increase in myofibers that contain higher numbers of satellite cells in both young and old rats, and an increase in the percent of myogenic clones derived from old rats. Changes at the satellite cell level in old rats were accompanied with positive effects on the lean-to-fat Gast muscle composition and on spontaneous locomotion levels. The significance of these data is that they suggest that the endurance exercise-mediated boost in both satellite numbers and myogenic properties may improve myofiber maintenance in aging.
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影响因子：
2.7
作者：
Day, Kenneth;Shefer, Gabi;Yablonka-Reuveni, Zipora
通讯作者：
Yablonka-Reuveni, Zipora
影响因子：
5.2
作者：
Collins, Charlotte A.;Zammit, Peter S.;Partridge, Terence A.
通讯作者：
Partridge, Terence A.
影响因子：
3.3
作者：
Enns, Deborah L.;Tiidus, Peter M.
通讯作者：
Tiidus, Peter M.
影响因子：
64.8
作者：
Conboy, IM;Conboy, MJ;Rando, TA
通讯作者：
Rando, TA
影响因子：
3.9
作者：
Deschenes, Michael R.;Roby, Mackenzie A.;Eason, Margaret K.;Harris, M. Brennan
通讯作者：
Harris, M. Brennan