Remodeling of the neuromuscular junction precedes sarcopenia related alterations in myofibers.
Remodeling of the neuromuscular junction precedes sarcopenia related alterations in myofibers.
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DOI：
10.1016/j.exger.2010.03.007
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发表时间：
2010-05
影响因子：
3.9
通讯作者：
Harris, M. Brennan
中科院分区：
文献类型：
--
作者：
Deschenes, Michael R.;Roby, Mackenzie A.;Eason, Margaret K.;Harris, M. Brennan
已经假设了导致肌肉减少症（与年龄相关的肌肉大小损失）的病因学的几种机制。其中之一是将肌肉质量的损失归因于先前与年龄相关的肌纤维去神经支配。本研究的目的是确定在纤维萎缩或纤维类型转换之前，神经肌肉接头（NMJ）处的去神经支配迹象是否明显，并揭示肌肉的活动水平是否影响其对年龄相关去神经支配的敏感性。跖肌和比目鱼肌取自年轻成年（10个月）和早期老年（21个月）大鼠。前和突触后NMJ形态定量与神经终末分支和终板区域的细胞荧光染色，分别。肌纤维概况（纤维大小和纤维类型组成）进行了评估与组织化学程序。结果表明，老年大鼠轻度募集的跖肌出现明显的失神经支配（P < 0.05），而相同肌肉的肌纤维轮廓无明显变化。然而，在大量招募的比目鱼肌中，几乎没有去神经支配的证据，肌纤维轮廓也没有改变。这些结果表明，年龄相关的去神经发生在肌纤维萎缩之前，大量的神经肌肉活动可能会延迟年龄相关的去神经和肌肉减少症的发生。
Several mechanisms contributing to the etiology of sarcopenia (age-related loss of muscle size) have been postulated. One of these attributes the loss of muscle mass to a preceding age-related denervation of myofibers. The aim of this study was to determine if signs of denervation were apparent at the neuromuscular junction (NMJ) before fiber atrophy, or fiber type conversion could be documented, and to reveal if a muscle’s activity level impacts its sensitivity to age-related denervation. Plantaris and soleus muscles were obtained from young adult (10 mo) and early aged (21 mo) rats. Pre- and post-synaptic NMJ morphology was quantified with cytofluorescent staining of nerve terminal branches and endplate regions, respectively. Myofiber profiles (fiber size and fiber type composition) were assessed with histochemical procedures. Results show that in the lightly recruited plantaris, significant (P < 0.05) signs of denervation were noted in aged rats, while the same muscles displayed no change in myofiber profile. In the heavily recruited soleus, however, there was little evidence of denervation, and again no alterations in myofiber profile. These results indicate that age-related denervation occurs before myofiber atrophy, and that high amounts of neuromuscular activity may delay the onset of age-related denervation and sarcopenia.
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DOI：
10.1093/gerona/54.11.b492
发表时间：
1999-11-01
影响因子：
5.1
作者：
Turturro, A;Witt, WW;Hart, RW
通讯作者：
Hart, RW
影响因子：
5.5
作者：
Payne, AM;Zheng, ZL;Delbono, O
通讯作者：
Delbono, O
影响因子：
--
作者：
LAUGHLIN, MH;ARMSTRONG, RB
通讯作者：
ARMSTRONG, RB
影响因子：
3.3
作者：
Delp, MD;Duan, CP
通讯作者：
Duan, CP
影响因子：
5.5
作者：
ELDRIDGE, L;LIEBHOLD, M;STEINBACH, JH
通讯作者：
STEINBACH, JH