Characterization of ciliated bronchial epithelium 1, a ciliated cell-associated gene induced during mucociliary differentiation.
Characterization of ciliated bronchial epithelium 1, a ciliated cell-associated gene induced during mucociliary differentiation.
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纤毛支气管上皮 1 的表征，这是粘膜纤毛分化过程中诱导的纤毛细胞相关基因。
DOI：
10.1165/rcmb.2004-0050oc
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发表时间：
2004
影响因子：
6.4
通讯作者：
J. Holloway
中科院分区：
文献类型：
--
作者：
H. Yoshisue;S. Puddicombe;S. Wilson;H. Haitchi;R. Powell;David I. Wilson;A. Pandit;Ann Berger;D. Davies;S. Holgate;J. Holloway
哮喘患者肺上皮结构改变；然而，上皮细胞修复的确切机制，包括从基底上皮细胞向柱状上皮细胞的分化，尚不清楚。在对人肺活检的cDNA文库进行随机测序的过程中，我们发现了一个新的基因，纤毛支气管上皮1 （CBE1）。CBE1在支气管上皮细胞体外分化过程中被诱导表达。与tektin和肝细胞核因子3/叉头同源物4同步表达，被白细胞介素-13下调，其组织分布强烈提示CBE1与纤毛细胞有关。经鉴定，该cDNA全长为0.7 kb，具有两个不同羧基末端的开放阅读框，与已知蛋白无同源性。免疫组化证实CBE1在纤毛上皮细胞中的表达。支气管活检定量逆转录聚合酶链反应分析显示CBE1在正常受试者和哮喘患者之间的表达无差异。表达研究表明，CBE1是核或核周定位，取决于细胞类型。CBE1在分化过程中的表达调控和亚细胞定位表明，它可能在人气道纤毛细胞的分化和/或功能中发挥重要作用。
Lung epithelial structure is altered in asthma; however, the precise mechanisms underlying epithelial repair, including differentiation from basal to columnar epithelial cells, are not well defined. In the course of random sequencing of a cDNA library from human lung biopsies, we have identified a novel gene, ciliated bronchial epithelium 1 (CBE1). Expression of CBE1 was induced during in vitro differentiation of bronchial epithelial cells. Synchronous expression with tektin and hepatocyte nuclear factor 3/forkhead homologue 4, down-regulation by interleukin-13, and its tissue distribution strongly suggested that CBE1 is associated with ciliated cells. Two isoforms of the 0.7-kb full-length cDNA were identified, resulting in open reading frames with different carboxyl termini, with no homology to known proteins. Expression of CBE1 in ciliated epithelial cells was confirmed by immunohistochemistry. Quantitative reverse transcription-polymerase chain reaction analysis using bronchial biopsies showed no difference of expression of CBE1 between normal subjects and subjects with asthma. Expression studies showed that CBE1 is nuclear- or perinuclear-localized, depending on cell type. Regulated expression during differentiation and the subcellular localization of CBE1 suggest that it may play an important role in the differentiation and/or function of ciliated cells in human airways.
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气道上皮纤毛发生早期表达的轴丝动力蛋白重链的表征。
DOI：
10.1165/ajrcmb.23.6.4045
发表时间：
2000
期刊：
American journal of respiratory cell and molecular biology.
影响因子：
--
作者：
Reed,W;Carson,JL;Moats-Staats,BM;Lucier,T;Hu,P;Brighton,L;Gambling,TM;Huang,CH;Leigh,MW;Collier,AM
通讯作者：
Collier,AM
DOI：
10.1165/ajrcmb.20.4.3442
发表时间：
1999-04-01
影响因子：
6.4
作者：
Bernacki, SH;Nelson, AL;Randell, SH
通讯作者：
Randell, SH
DOI：
10.1165/ajrcmb.21.2.3691
发表时间：
1999-08-01
影响因子：
6.4
作者：
Blatt, EN;Yan, XH;Brody, SL
通讯作者：
Brody, SL
DOI：
10.1164/ajrccm.163.2.2004039
发表时间：
2001-02-01
影响因子：
24.7
作者：
Ordoñez, CL;Khashayar, R;Fahy, JV
通讯作者：
Fahy, JV