The development of novel bioactive porous titanium as a bone reconstruction material.
The development of novel bioactive porous titanium as a bone reconstruction material.
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作者：
树脂钛替代技术制备的多孔钛具有良好的机械强度和骨传导性。钛表面的碱处理产生生物活性表面。碱处理的多孔钛有望加速骨形成。本研究的目的是评价碱处理多孔钛的骨重建能力。用碱溶液（5 N NaOH，24小时）处理多孔钛（85%孔隙率）。为了评估材料性能，我们通过扫描电子显微镜（SEM）和机械强度测试分析了表面结构。为了评估生物活性，将每个样品浸泡在模拟体液（Hank溶液）中7天。进行表面观察、重量变化率测量（在Hank溶液中浸泡之后/之前）和表面元素分析。我们还设计了一个兔股骨的体内研究。植入2周和3周后，进行组织学观察和组织形态计量学骨形成率分析。使用Student's t检验（P < 0.05）对所有数据进行统计学分析（该研究由广岛大学动物实验伦理委员会批准：A11-5-5）。未处理的多孔钛（对照）似乎具有光滑的表面，碱处理的多孔钛（ATPT）具有纳米尺寸的针状粗糙表面。ATPT具有与对照相似的机械强度。浸泡到Hank溶液中后，我们在SEM图像中观察到磷灰石样晶体，重量增加，以及ATPT中的高Ca和P含量。与对照组相比，ATPT组早期有明显的骨形成。结果表明，碱处理后的多孔钛表面具有生物活性，能有效诱导骨重建。这种新型生物活性多孔钛有望成为一种良好的骨重建材料。树脂钛替代技术制备的多孔钛具有良好的机械强度和骨传导性。
Porous titanium fabricated by the resin-impregnated titanium substitute technique has good mechanical strength and osteoconduction. The alkali treatment of the titanium surface creates a bioactive surface. Alkali-treated porous titanium is expected to accelerate bone formation. The purpose of this study was to evaluate the bone reconstruction ability of alkali-treated porous titanium. Porous titanium (85% porosity) was treated with an alkali solution (5 N NaOH, 24 h). To assess material properties, we analyzed the surface structure by scanning electron microscopy (SEM) and mechanical strength testing. To assess bioactivity, each sample was soaked in a simulated body fluid (Hank's solution) for 7 days. Surface observations, weight change ratio measurement (after/before being soaked in Hank's solution) and surface elemental analysis were performed. We also designed an in vivo study with rabbit femurs. After 2 and 3 weeks of implantation, histological observations and histomorphometric bone formation ratio analysis were performed. All data were statistically analyzed using a Student's t-test (P < 0.05) (this study was approved by the Hiroshima University animal experiment ethics committee: A11-5-5). Non-treated porous titanium (control) appeared to have a smooth surface and the alkali-treated porous titanium (ATPT) had a nano-sized needle-like rough surface. ATPT had similar mechanical strength to that of the control. After soaking into the Hank's solution, we observed apatite-like crystals in the SEM image, weight gain, and high Ca and P contents in ATPT. There was significant bone formation at an early stage in ATPT compared with that in control. It was suggested that the alkali-treated porous titanium had a bioactive surface and induced bone reconstruction effectively. This novel bioactive porous titanium can be expected to be a good bone reconstruction material. Porous titanium fabricated by the resin-impregnated titanium substitute technique has good mechanical strength and osteoconduction.
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影响因子：
3.9
作者：
Kobatake, Reiko;Doi, Kazuya;Kubo, Takayasu;Makihara, Yusuke;Oki, Yoshifumi;Yokoi, Miyuki;Umehara, Hanako;Tsuga, Kazuhiro
通讯作者：
Tsuga, Kazuhiro
影响因子：
4.3
作者：
Chang, Chin-Shan;Lee, Tzer-Min;Liu, Jia-Kuang
通讯作者：
Liu, Jia-Kuang
影响因子：
0.9
作者：
Ferretti, C;Ripamonti, U
通讯作者：
Ripamonti, U
DOI：
10.1002/jbm.10194
发表时间：
2002-07-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Serro, AP;Fernandes, AC;Fernandes, MHV
通讯作者：
Fernandes, MHV
影响因子：
14
作者：
Feng, Ya-Fei;Wang, Lin;Zhang, Zhi-Yong
通讯作者：
Zhang, Zhi-Yong