A quantum dot microspheres-based highly specific and sensitive three-dimensional microarray for multiplexed detection of inflammatory factors
A quantum dot microspheres-based highly specific and sensitive three-dimensional microarray for multiplexed detection of inflammatory factors
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基于量子点微球的高特异性和灵敏的三维微阵列用于炎症因子的多重检测
DOI：
10.1088/1361-6528/ac1bdd
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发表时间：
2021-08
期刊：
影响因子：
3.5
通讯作者：
Lin Song Li
中科院分区：
文献类型：
--
作者：
Yanbing Lv;Hongke Xu;Ruili Wu;Yanxia Xu;Ning Li;Jinjie Li;Huaibin Shen;Hongwei Ma;Fang Guo;Lin Song Li
体外诊断学的发展趋势是以极低的浓度和体积从一个样本中获得各种生物信息，这推动了其在准确和灵敏的多重检测方面的进步。在这里，我们研制了一种基于单色量子点(QD)的三维(3D)结构矩阵微阵列，并利用自行构建的荧光检测系统对两种炎症因子(C-反应蛋白(CRP)和血清淀粉样蛋白A(SAA))进行了检测。这一策略通过将抗体特定地固定在3D底物上来提高检测灵敏度，因为与二维(2D)平面相比，它捕获了大约7倍的“有效”抗体。与双量子点-2D荧光连接免疫吸附分析相比，基于两亲性聚合物修饰量子点的三维微阵列的检测限进一步提高，分别为0.11ngmlCRP1和0.16ngmlCRP1。采用QD微球(SiO_2@Qds@SiO_2-COOH，每个SiO_2球上含有约200-300个疏水量子点)作为荧光标记物，3D微阵列对C反应蛋白和SA的检测限分别达到15pgml−1和86pgml−1，灵敏度分别提高28倍和425倍。由于其优异的性能，这种基于QD微球的三维微阵列在生物医学和食品安全领域中对其他生物标志物、小分子和抗生素残留的高灵敏度和多重定量检测具有巨大的应用潜力。
The development trend of in vitro diagnostics is to obtain various biological information from a sample at extremely low concentration and volume, which has promoted its progress in accurate and sensitive multiplexed detection. Here, we developed a single color quantum dot (QD) based three-dimensional (3D) structure matrix microarray and conducted the detection of two inflammatory factors (C-reactive protein (CRP) and serum amyloid A (SAA)) by a self-built fluorescence detection system. This strategy increased detection sensitivity by immobilizing the antibody specifically on the 3D substrate because it captured more than about 7 times of ‘effective’ antibodies compared to the two-dimensional (2D) plane. Compared to the dual QDs-2D fluorescence-linked immunosorbent assay, the limit of detection (LOD) of 3D microarray based on QDs modified with amphiphilic polymers has been further improved to 0.11 ng ml−1 for SAA assay and to 0.16 ng ml−1 for CRP assay, respectively. By using QD microspheres (SiO2@QDs@SiO2-COOH, containing approximately 200–300 hydrophobic QDs on per SiO2 sphere) as fluorescent labels, the LOD for CRP and SAA of 3D microarray reached as high as 15 pg ml−1 and 86 pg ml−1, and the sensitivity was further improved by 28-fold and 425-fold, respectively. Because of its excellent performance, this QD microspheres-based 3D microarray has great application potential for highly sensitive and multiplexed quantitative detection of other biomarkers, small molecules, and antibiotic residues in biomedicine and food safety.
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