Subterranean, herbivore-induced plant volatile increases biological control activity of multiple beneficial nematode species in distinct habitats.
Subterranean, herbivore-induced plant volatile increases biological control activity of multiple beneficial nematode species in distinct habitats.
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DOI：
10.1371/journal.pone.0038146
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Stelinski LL
中科院分区：
文献类型：
--
作者：
Ali JG;Alborn HT;Campos-Herrera R;Kaplan F;Duncan LW;Rodriguez-Saona C;Koppenhöfer AM;Stelinski LL
虽然草食动物诱导的挥发物在植物-草食动物-天敌相互作用中的作用已经得到了很好的证明，但新的证据表明，地下挥发性物质的排放可以通过吸引昆虫病原线虫(EPN)来保护植物。然而，由于方法的限制，以前还没有研究在田间发现地下食草动物诱导的挥发物或量化它们对不同EPN物种吸引的影响。在这里，我们展示了地下食草动物诱导的挥发性物质如何提高农业上重要的根部害虫的死亡率。首先，我们实时地从柑橘幼虫取食后9-12小时的根中鉴定出了前吉耶烯(1，5-二甲基环十-1，5，7-三烯)。在田间成熟的柑橘树的根部也检测到了这种化合物。在柑橘园中，应用从被象虫危害的柑橘根收集的挥发物，吸引了当地的EPN，并增加了甲虫幼虫的死亡率。此外，分离的前季青烯的现场应用也产生了类似的结果。实时定量聚合酶链式反应显示，前吉耶烯通过在田间吸引四种自然产生的EPN而增加了害虫的死亡率。最后，我们通过在蓝莓田里应用PregeiJerene来测试这种根区信号的普遍性；通过吸引EPN的自然发生种群，幼虫(Galleria mellonella和omala orientalis)的死亡率再次增加。因此，这种特殊的地下信号吸引了不同农业系统中广泛存在的根害虫的天敌，并可能在根害虫的生物防治方面具有广阔的潜力。
While the role of herbivore-induced volatiles in plant-herbivore-natural enemy interactions is well documented aboveground, new evidence suggests that belowground volatile emissions can protect plants by attracting entomopathogenic nematodes (EPNs). However, due to methodological limitations, no study has previously detected belowground herbivore-induced volatiles in the field or quantified their impact on attraction of diverse EPN species. Here we show how a belowground herbivore-induced volatile can enhance mortality of agriculturally significant root pests. First, in real time, we identified pregeijerene (1,5-dimethylcyclodeca-1,5,7-triene) from citrus roots 9–12 hours after initiation of larval Diaprepes abbreviatus feeding. This compound was also detected in the root zone of mature citrus trees in the field. Application of collected volatiles from weevil-damaged citrus roots attracted native EPNs and increased mortality of beetle larvae (D. abbreviatus) compared to controls in a citrus orchard. In addition, field applications of isolated pregeijerene caused similar results. Quantitative real-time PCR revealed that pregeijerene increased pest mortality by attracting four species of naturally occurring EPNs in the field. Finally, we tested the generality of this root-zone signal by application of pregeijerene in blueberry fields; mortality of larvae (Galleria mellonella and Anomala orientalis) again increased by attracting naturally occurring populations of an EPN. Thus, this specific belowground signal attracts natural enemies of widespread root pests in distinct agricultural systems and may have broad potential in biological control of root pests.
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影响因子：
2.3
作者：
Ali, Jared G.;Alborn, Hans T.;Stelinski, Lukasz L.
通讯作者：
Stelinski, Lukasz L.
影响因子：
1.4
作者：
Bullock, RC;Pelosi, RR;Killer, EE
通讯作者：
Killer, EE
DOI：
10.1007/978-3-642-12162-3_12
发表时间：
2010-01-01
期刊：
PLANT COMMUNICATION FROM AN ECOLOGICAL PERSPECTIVE
影响因子：
--
作者：
Bruce, Toby J. A.
通讯作者：
Bruce, Toby J. A.
DOI：
10.1073/pnas.160241697
发表时间：
2000-08-01
影响因子：
11.1
作者：
Birkett, MA;Campbell, CAM;Woodcock, CM
通讯作者：
Woodcock, CM
影响因子：
56.9
作者：
GREEN, TR;RYAN, CA
通讯作者：
RYAN, CA