Lactobacillus plantarum displaying conserved M2e and HA2 fusion antigens induces protection against influenza virus challenge
Lactobacillus plantarum displaying conserved M2e and HA2 fusion antigens induces protection against influenza virus challenge
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展示保守 M2e 和 HA2 融合抗原的植物乳杆菌诱导针对流感病毒攻击的保护
DOI：
10.1007/s00253-018-8924-6
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
王春凤
中科院分区：
文献类型：
--
作者：
杨文涛;杨桂连;赵亮;晋玉北;姜延龙;黄海斌;石春卫;王建忠;王冠;康元环;王春凤
禽流感病毒（AIV）可感染禽类、哺乳动物及其他宿主，并给全球家禽业造成巨大经济损失。在本研究中，为了开发一种基于植物乳杆菌（L. plantarum）的新型高效口服疫苗用于……
Avian influenza virus (AIV) can infect poultry, mammals, and other hosts and causes enormous economic losses to the global poultry industry. In this study, to develop a novel and potent oral vaccine based on Lactobacillus plantarum (L. plantarum) for controlling the spread of AIV in the poultry industry, we constructed a recombinant L. plantarum strain displaying the 3M2e-HA2 protein of the influenza virus and determined the effect of N/pgsA'-3M2e-HA2 against AIV in chicks. We first confirmed that the 3M2e-HA2 fusion protein was expressed on the surface of L. plantarum via flow cytometry and immunofluorescence experiments. Our experimental results demonstrated that chicks immunized with N/pgsA'-3M2e-HA2 could induce specific humoral, mucosal, and T cell-mediated immune responses, eliciting the host body to protect itself against AIV. Additionally, compared to oral administration, the intranasal immunization of chicks with N/pgsA'-3M2e-HA2 provided a stronger immune response, resulting in a potent protective effect that hindered the loss of body weight, decreasing pulmonary virus titers and reducing lung and throat pathological damages. Thus, our results indicate that our novel approach is an effective method of vaccine design to promote mucosal immunity.
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