Identification of key genes and pathways in regulating immune‑induced diseases of dendritic cells by bioinformatic analysis.
Identification of key genes and pathways in regulating immune‑induced diseases of dendritic cells by bioinformatic analysis.
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通过生物信息分析鉴定调节树突状细胞免疫诱导疾病的关键基因和通路
DOI：
10.3892/mmr.2018.8834
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发表时间：
2018-06
影响因子：
3.4
通讯作者：
Yu B
中科院分区：
文献类型：
--
作者：
Zheng Y;Zheng X;Li S;Zhang H;Liu M;Yang Q;Zhang M;Sun Y;Wu J;Yu B
树突状细胞（Dendritic cells，DC）在免疫应答的激活中起重要作用，DC激活或抑制的失衡与免疫诱导疾病易感性的增加有关。然而，DCs调控免疫诱导疾病的分子机制还不清楚。本研究的目的是鉴定DCs中的基因特征并揭示其潜在的调控机制。共整合并深入分析了4个基因表达谱（GSE 52894、GSE 72893、GSE 75938和GSE 77969）。总共检测到241个上调基因和365个下调基因。基因本体和途径富集分析显示，差异表达基因（DEG）在炎症反应、肿瘤坏死因子（TNF）信号通路、核因子（NF）-κB信号通路和抗原加工等方面明显富集。从蛋白质-蛋白质分析中鉴定了前10个枢纽基因。从蛋白质-蛋白质网络中过滤出最重要的2个模块。2个模块中的基因分别参与I型干扰素信号通路、NF-κB信号通路和TNF信号通路。此外，进行microRNA-mRNA网络分析。本研究的结果表明，所鉴定的DEG和通路可以提高我们对DCs成熟机制的理解，以及可能成为免疫诱导疾病治疗靶点的候选枢纽基因。
Dendritic cells (DCs) serve crucial roles in the activation of the immune response, and imbalance in the activation or inhibition of DCs has been associated with an increased susceptibility to develop immune-induced diseases. However, the molecular mechanisms of regulating immune-induced diseases of DCs are not well understood. The aim of the present study was to identify the gene signatures and uncover the potential regulatory mechanisms in DCs. A total of 4 gene expression profiles (GSE52894, GSE72893, GSE75938 and GSE77969) were integrated and analyzed in depth. In total, 241 upregulated genes and 365 downregulated genes were detected. Gene ontology and pathway enrichment analysis showed that the differentially expressed genes (DEGs) were significantly enriched in the inflammatory response, the tumor necrosis factor (TNF) signaling pathway, the nuclear factor (NF)-κB signaling pathway and antigen processing. The top 10 hub genes were identified from the protein-protein analysis. The most significant 2 modules were filtered from the protein-protein network. The genes in 2 modules were involved in type I interferon signaling, the NF-κB signaling pathway and the TNF signaling pathway. Furthermore, the microRNA-mRNA network analysis was performed. The results of the present study revealed that the identified DEGs and pathways may improve our understanding of the mechanisms of the maturation of DCs, and the candidate hub genes that may be therapeutic targets for immune-induced diseases.
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影响因子：
3.3
作者：
Haudek-Prinz VJ;Klepeisz P;Slany A;Griss J;Meshcheryakova A;Paulitschke V;Mitulovic G;Stöckl J;Gerner C
通讯作者：
Gerner C
USP18的多个功能。
影响因子：
9
作者：
Honke N;Shaabani N;Zhang DE;Hardt C;Lang KS
通讯作者：
Lang KS
影响因子：
5.5
作者：
Hu, Yang;Park-Min, Kyung-Hyun;Ivashkiv, Lionel B.
通讯作者：
Ivashkiv, Lionel B.
影响因子：
4.4
作者：
Chae, Chang-Suk;Kim, Gi-Cheon;Im, Sin-Hyeog
通讯作者：
Im, Sin-Hyeog
影响因子：
5.5
作者：
Malinova D;Fritzsche M;Nowosad CR;Armer H;Munro PM;Blundell MP;Charras G;Tolar P;Bouma G;Thrasher AJ
通讯作者：
Thrasher AJ