Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy.
Effects of Diabetes on Microcirculation and Leukostasis in Retinal and Non-Ocular Tissues: Implications for Diabetic Retinopathy.
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DOI：
10.3390/biom10111583
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发表时间：
2020-11-21
期刊：
影响因子：
5.5
通讯作者：
Roy S
中科院分区：
文献类型：
--
作者：
Herdade AS;Silva IM;Calado Â;Saldanha C;Nguyen NH;Hou I;Castanho M;Roy S
视网膜微循环的改变与糖尿病视网膜病变（DR）的发展有关。然而，目前尚不清楚这种变化是否也发生在其他非视网膜组织的毛细血管床。在这里，我们研究了微循环的变化，包括速度滚动中性粒细胞，粘附的中性粒细胞，白细胞淤滞在发展过程中的视网膜血管病变的糖尿病在其他非视网膜组织。活体显微镜下观察了重度或中度糖尿病小鼠提睾肌和耳垂的毛细血管后小静脉，并与非糖尿病小鼠进行了比较。此外，定量滚动白细胞的数量和速度、粘附白细胞的数量和白细胞停滞的面积，并检查视网膜毛细血管网络的无细胞毛细血管（AC）和周细胞损失（PL），糖尿病小鼠的提睾肌和耳垂毛细血管后小静脉中粘附的中性粒细胞的数量和白细胞停滞的区域与非糖尿病小鼠相比增加。糖尿病小鼠同样，与非糖尿病对照小鼠相比，观察到滚动中性粒细胞数量显著增加，滚动速度降低，糖尿病的严重程度加剧了这些变化。了解糖尿病引起的提睾肌和耳垂的微循环变化可能有助于深入了解DR中视网膜血管病变的发展。
Changes in retinal microcirculation are associated with the development of diabetic retinopathy (DR). However, it is unclear whether such changes also develop in capillary beds of other non-retinal tissues. Here, we investigated microcirculatory changes involving velocity of rolling neutrophils, adherence of neutrophils, and leukostasis during development of retinal vascular lesions in diabetes in other non-retinal tissues. Intravital microscopy was performed on post-capillary venules of cremaster muscle and ear lobe of mice with severe or moderate diabetes and compared to those of non-diabetic mice. Additionally, number and velocity of rolling leukocytes, number of adherent leukocytes, and areas of leukostasis were quantified, and retinal capillary networks were examined for acellular capillaries (AC) and pericyte loss (PL), two prominent vascular lesions characteristic of DR. The number of adherent neutrophils and areas of leukostasis in the cremaster and ear lobe post-capillary venules of diabetic mice was increased compared to those of non-diabetic mice. Similarly, a significant increase in the number of rolling neutrophils and decrease in their rolling velocities compared to those of non-diabetic control mice were observed and severity of diabetes exacerbated these changes. Understanding diabetes-induced microcirculatory changes in cremaster and ear lobe may provide insight into retinal vascular lesion development in DR.
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影响因子：
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