Elk3 is essential for the progression from progenitor to definitive neural crest cell.
Elk3 is essential for the progression from progenitor to definitive neural crest cell.
复制标题
ELK3对于从祖细胞到确定的神经rest细胞的发展至关重要。
DOI：
10.1016/j.ydbio.2012.12.009
复制
发表时间：
2013-02-15
影响因子：
2.7
通讯作者：
Bronner, Marianne E.
中科院分区：
文献类型：
--
作者：
Rogers, Crystal D.;Phillips, Jacquelyn L.;Bronner, Marianne E.
Elk3/Net/Sap2（此处称为Elk3）是一种Ets三元复合转录抑制因子，因其参与胚胎发育过程中的血管生成而闻名。尽管Elk3在多种组织中表达，但该蛋白在脉管系统发育之外的其他作用尚未有报道。在这里，我们利用整体原位杂交和定量 RT-PCR 表征了鸟类胚胎中 Elk3 的早期时空表达模式，并检查了其功能丧失对神经嵴发育的影响。在早期阶段，Elk3 在头部皱襞、头部间充质、体节间血管和迁移性颅神经嵴 (NC) 细胞中表达。 Elk3蛋白的缺失会导致Pax7+前体细胞保留在背侧神经管中，而这些前体细胞无法上调神经嵴特异性基因FoxD3、Sox10和Snail2，从而导致胚胎出现严重的迁移缺陷。结果推测，Elk3位于神经板边界基因的下游，但位于神经嵴基因调控网络（NC-GRN）中神经嵴特异性基因的上游，这表明它对于从祖细胞到最终神经嵴细胞的进展至关重要。
Elk3/Net/Sap2 (here referred to as Elk3) is an Ets ternary complex transcriptional repressor known for its involvement in angiogenesis during embryonic development. Although Elk3 is expressed in various tissues, additional roles for the protein outside of vasculature development have yet to be reported. Here, we characterize the early spatiotemporal expression pattern of Elk3 in the avian embryo using whole mount in situ hybridization and quantitative RT-PCR and examine the effects of its loss of function on neural crest development. At early stages, Elk3 is expressed in the head folds, head mesenchyme, intersomitic vessels, and migratory cranial neural crest (NC) cells. Loss of the Elk3 protein results in the retention of Pax7+ precursors in the dorsal neural tube that fail to upregulate neural crest specifier genes, FoxD3, Sox10 and Snail2, resulting in embryos with severe migration defects. The results putatively place Elk3 downstream of neural plate border genes, but upstream of neural crest specifier genes in the neural crest gene regulatory network (NC-GRN), suggesting that it is critical for the progression from progenitor to definitive neural crest cell.
登录
查看更多内容
影响因子：
3.1
作者：
Nozaki, M;Onishi, Y;Fujimura, Y
通讯作者：
Fujimura, Y
影响因子：
10.5
作者：
Zheng, H;Wasylyk, C;Wasylyk, B
通讯作者：
Wasylyk, B
影响因子：
3.7
作者：
Murdoch B;DelConte C;García-Castro MI
通讯作者：
García-Castro MI
影响因子：
64.8
作者：
Basch, ML;Bronner-Fraser, M;García-Castro, MI
通讯作者：
García-Castro, MI
影响因子：
4.6
作者：
Drerup, Catherine M.;Wiora, Heather M.;Morris, Jill A.
通讯作者：
Morris, Jill A.