Pax7 lineage contributions to the mammalian neural crest.
Pax7 lineage contributions to the mammalian neural crest.
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DOI：
10.1371/journal.pone.0041089
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
García-Castro MI
中科院分区：
文献类型：
--
作者：
Murdoch B;DelConte C;García-Castro MI
神经嵴细胞是脊椎动物特有的多能细胞，对多种组织有贡献，包括周围神经系统、黑素细胞、颅面骨和软骨。神经嵴发育异常与多种人类疾病有关，包括腭裂/唇腭裂、黑色素瘤和神经母细胞瘤等侵袭性癌症，以及瓦登堡综合征等罕见综合征，这是一种涉及听力损失和色素缺陷的复杂疾病。我们之前将转录因子 Pax7 鉴定为早期标记物，并且是鸡胚胎神经嵴发育所需的成分。在哺乳动物中，Pax7 也被认为在神经嵴发育中发挥作用，但 Pax7 祖细胞对神经嵴谱系的精确贡献尚未确定。在这里，我们在双转基因小鼠中使用 Cre/loxP 技术来绘制神经嵴衍生物中 Pax7 谱系的命运图谱。我们发现 Pax7 后代对多种组织有贡献，包括颅神经嵴、心脏神经嵴和躯干神经嵴，这些组织在颅软骨中形成独特的区域模式。与 Pax3 谱系一样，Pax7 谱系也在一些非神经嵴组织中检测到，包括特定器官中的上皮细胞子集。这些结果表明 Pax7 后代在神经嵴内外广泛分布，这一点以前未被认识到。它们揭示了在 Pax3 和 Pax7 突变体中观察到的区域不同表型，并为 Pax7 在疾病和发育过程中的潜在作用提供了独特的视角。
Neural crest cells are vertebrate-specific multipotent cells that contribute to a variety of tissues including the peripheral nervous system, melanocytes, and craniofacial bones and cartilage. Abnormal development of the neural crest is associated with several human maladies including cleft/lip palate, aggressive cancers such as melanoma and neuroblastoma, and rare syndromes, like Waardenburg syndrome, a complex disorder involving hearing loss and pigment defects. We previously identified the transcription factor Pax7 as an early marker, and required component for neural crest development in chick embryos. In mammals, Pax7 is also thought to play a role in neural crest development, yet the precise contribution of Pax7 progenitors to the neural crest lineage has not been determined. Here we use Cre/loxP technology in double transgenic mice to fate map the Pax7 lineage in neural crest derivates. We find that Pax7 descendants contribute to multiple tissues including the cranial, cardiac and trunk neural crest, which in the cranial cartilage form a distinct regional pattern. The Pax7 lineage, like the Pax3 lineage, is additionally detected in some non-neural crest tissues, including a subset of the epithelial cells in specific organs. These results demonstrate a previously unappreciated widespread distribution of Pax7 descendants within and beyond the neural crest. They shed light regarding the regionally distinct phenotypes observed in Pax3 and Pax7 mutants, and provide a unique perspective into the potential roles of Pax7 during disease and development.
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影响因子：
1.5
作者：
Lepper, Christoph;Fan, Chen-Ming
通讯作者：
Fan, Chen-Ming
影响因子：
2.6
作者：
JOSTES, B;WALTHER, C;GRUSS, P
通讯作者：
GRUSS, P
影响因子：
--
作者：
AUERBACH, R
通讯作者：
AUERBACH, R
DOI：
10.1073/pnas.90.2.532
发表时间：
1993-01-15
影响因子：
11.1
作者：
EPSTEIN, DJ;VOGAN, KJ;GROS, P
通讯作者：
GROS, P
影响因子：
64.8
作者：
Basch, ML;Bronner-Fraser, M;García-Castro, MI
通讯作者：
García-Castro, MI