The genomic basis of copper tolerance in Drosophila is shaped by a complex interplay of regulatory and environmental factors.
The genomic basis of copper tolerance in Drosophila is shaped by a complex interplay of regulatory and environmental factors.
复制标题
DOI：
10.1186/s12915-022-01479-w
复制
发表时间：
2022-12-08
期刊：
影响因子：
5.4
通讯作者：
González J
中科院分区：
文献类型：
--
作者：
Green L;Coronado-Zamora M;Radío S;Rech GE;Salces-Ortiz J;González J
工业化和人类活动的升级导致排放到环境中的污染物增加。在这些污染物中，重金属如铜由于其生物累积性质而特别令人关注。由于其高度异质性的分布和作为必需微量营养素和有毒元素的双重性质，铜耐受性的遗传基础可能是由遗传和环境因素的复杂相互作用形成的。在这项研究中，我们利用在欧洲收集的多个种群的果蝇中存在的自然变异来筛选铜耐受性的变异。我们发现，纬度和采集地的城市化程度，而不是任何其他环境因素的组合，与铜耐受性有关。虽然先前确定的铜相关基因在耐受与敏感菌株中没有差异表达，但参与代谢、繁殖和蛋白酶诱导的基因促成了差异胁迫反应。此外，在中肠中观察到对铜毒性的最大转录组学和生理学反应，我们发现肠道酸度的保持与更大的耐受性密切相关。最后，我们确定了转座因子插入可能在铜胁迫反应中发挥作用。总的来说，通过将全基因组方法与环境关联分析和候选基因的功能分析相结合，我们的研究提供了一个独特的视角，即在自然条件下形成铜耐受性的遗传和环境因素。黑腹果蝇种群，并确定新的基因，转座因子和生理特性参与这一复杂的表型。在线版本包含补充材料，可通过10.1186/s12915-022-01479-w获得。
Escalation in industrialization and anthropogenic activity have resulted in an increase of pollutants released into the environment. Of these pollutants, heavy metals such as copper are particularly concerning due to their bio-accumulative nature. Due to its highly heterogeneous distribution and its dual nature as an essential micronutrient and toxic element, the genetic basis of copper tolerance is likely shaped by a complex interplay of genetic and environmental factors. In this study, we utilized the natural variation present in multiple populations of Drosophila melanogaster collected across Europe to screen for variation in copper tolerance. We found that latitude and the degree of urbanization at the collection sites, rather than any other combination of environmental factors, were linked to copper tolerance. While previously identified copper-related genes were not differentially expressed in tolerant vs. sensitive strains, genes involved in metabolism, reproduction, and protease induction contributed to the differential stress response. Additionally, the greatest transcriptomic and physiological responses to copper toxicity were seen in the midgut, where we found that preservation of gut acidity is strongly linked to greater tolerance. Finally, we identified transposable element insertions likely to play a role in copper stress response. Overall, by combining genome-wide approaches with environmental association analysis, and functional analysis of candidate genes, our study provides a unique perspective on the genetic and environmental factors that shape copper tolerance in natural D. melanogaster populations and identifies new genes, transposable elements, and physiological traits involved in this complex phenotype. The online version contains supplementary material available at 10.1186/s12915-022-01479-w.
登录
查看更多内容
影响因子：
4.5
作者：
Catalán A;Glaser-Schmitt A;Argyridou E;Duchen P;Parsch J
通讯作者：
Parsch J
影响因子：
5.6
作者：
Calap-Quintana P;González-Fernández J;Sebastiá-Ortega N;Llorens JV;Moltó MD
通讯作者：
Moltó MD
影响因子：
4.6
作者：
Denecke S;Fusetto R;Martelli F;Giang A;Battlay P;Fournier-Level A;O' Hair RA;Batterham P
通讯作者：
Batterham P
影响因子：
2.1
作者：
Arthur, A. L.;Weeks, A. R.;Sgro, C. M.
通讯作者：
Sgro, C. M.
影响因子：
11.4
作者：
Balamurugan, Kuppusamy;Egli, Dieter;Schaffner, Walter
通讯作者：
Schaffner, Walter