The effects of simulated microgravity on intervertebral disc degeneration.
The effects of simulated microgravity on intervertebral disc degeneration.
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DOI：
10.1016/j.spinee.2012.01.022
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发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Jin L;Feng G;Reames DL;Shimer AL;Shen FH;Li X
宇航员在太空飞行期间和之后会经历背痛，特别是腰痛。最近的研究描述了太空旅行后大鼠椎间盘的组织学和生化变化，但仍然没有体外模型来研究微重力对椎间盘代谢的影响。为研究微重力对椎间盘退变的影响，建立体外模拟微重力研究模型，在生物反应器中进行静态和旋转条件下的椎间盘培养，评价椎间盘退变的特征。在静态和微重力条件下培养椎间盘。进行组织学、生物化学和免疫组织化学测定以评价微重力条件下椎间盘的特征。发现在旋转生物反应器中培养的椎间盘发生椎间盘退变的变化，表现为纤维环内红色番红-o染色减少、GAG含量和GAG/Hypro比率下调、MMP-3表达增加和细胞凋亡上调。我们的结论是，模拟微重力诱导椎间盘退变的分子变化。旋转生物反应器模型为研究微重力对椎间盘代谢的影响提供了基础。
Astronauts experience back pain, particularly low back pain, during and after spaceflight. Recent studies have described histological and biochemical changes in rat intervertebral discs after space travel, but there is still no in vitro model to investigate the effects of microgravity on disc metabolism. To study the effects of microgravity on disc degeneration and to establish an in vitro simulated microgravity study model Discs were cultured in static and rotating conditions in bioreactor, and the characteristics of disc degeneration were evaluated The mice discs were cultured in a rotating wall vessel bioreactor where the microgravity condition was simulated. Intervertebral discs were cultured in static and microgravity condition. Histology, biochemistry, and immunohistochemical assays were performed to evaluate the characteristics of the discs in microgravity condition. Intervertebral discs cultured in rotating bioreactors were found to develop changes of disc degeneration manifested by reduced red Safranin-o staining within the annulus fibrosus, downregulated GAG content and GAG/Hypro ratio, increased MMP-3 expression, and upregulated apoptosis. We conclude that simulated microgravity induces the molecular changes of disc degeneration. The rotating bioreactor model will provide a foundation to investigate the effects of microgravity on disc metabolism.
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影响因子：
4.5
作者：
Cui, Min;Wan, Yuqing;Li, Xudong
通讯作者：
Li, Xudong
DOI：
10.1097/00005768-199608000-00007
发表时间：
1996-08-01
期刊：
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
影响因子：
--
作者：
Hargens, AR;Watenpaugh, DE
通讯作者：
Watenpaugh, DE
影响因子：
3
作者：
Hutton, WC;Elmer, WA;Hair, GA
通讯作者：
Hair, GA
影响因子：
15.9
作者：
Antoniou, J;Steffen, T;Alini, M
通讯作者：
Alini, M
影响因子：
3
作者：
Anderson, DG;Li, XD;Balian, G
通讯作者：
Balian, G