Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis).
Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis).
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大白菜 (Brassica Campestris L. ssp. pekinensis) 的全转录组分析和花药发育相关 ceRNA 网络的构建
DOI：
10.1038/s41598-022-06556-2
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发表时间：
2022-02-17
影响因子：
4.6
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Shi F;Pang Z;Liu C;Zhou L;Tan C;Ren J;Ye X;Feng H;Liu Z
花药发育受复杂的基因网络精确调控，这对植物育种具有重要意义。然而，大白菜花药发育的分子机制尚不清楚。本研究以大白菜（Brassica campestris L.）为材料，鉴定了与花药发育相关的microRNA（miRNAs）、mRNA、长链非编码RNA（lncRNA）和环状RNA（circRNA）。SSP. pekinensis）的研究，构建竞争性内源RNA（ceRNA）调控网络，为花药发育提供有价值的知识。利用全转录组测序技术，在大白菜花药中分别鉴定出9055、585、1344和165个差异表达的mRNA（DEmRNAs）、miRNAs（DEmiRNAs）、lncRNAs（DElncRNAs）和circRNAs（DECircRNAs）。利用miRNA靶向关系构建了花药相关ceRNA调控网络，共获得450对ceRNA关系，包括97对DEmiRNA-DEmRNA、281对DEmiRNA-DElncRNA和23对DEmiRNA-DEcircRNA相互作用。我们确定了参与小孢子发生、绒毡层和胼胝质层发育、花粉壁形成和花药开裂的重要基因及其与lncRNA、circRNA和miRNAs的相互作用。我们分析了在拟南芥花药中特异表达的六个花药优势表达基因的启动子活性，表明它们可能在大白菜花药发育中发挥重要作用。本研究为进一步研究大白菜花药生长发育的分子机制奠定了基础。
Anther development is precisely regulated by a complex gene network, which is of great significance to plant breeding. However, the molecular mechanism of anther development in Chinese cabbage is unclear. Here, we identified microRNAs (miRNAs), mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) related to anther development in Chinese cabbage (Brassica campestris L. ssp. pekinensis) to construct competitive endogenous RNA (ceRNA) regulatory networks and provide valuable knowledge on anther development. Using whole-transcriptome sequencing, 9055, 585, 1344, and 165 differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs), lncRNAs (DElncRNAs), and circRNAs (DEcircRNAs) were identified, respectively, in the anthers of Chinese cabbage compared with those in samples of the vegetative mass of four true leaves. An anther-related ceRNA regulatory network was constructed using miRNA targeting relationships, and 450 pairs of ceRNA relationships, including 97 DEmiRNA–DEmRNA, 281 DEmiRNA–DElncRNA, and 23 DEmiRNA–DEcircRNA interactions, were obtained. We identified important genes and their interactions with lncRNAs, circRNAs, and miRNAs involved in microsporogenesis, tapetum and callose layer development, pollen wall formation, and anther dehiscence. We analyzed the promoter activity of six predominant anther expression genes, which were expressed specifically in the anthers of Arabidopsis thaliana, indicating that they may play an important role in anther development of Chinese cabbage. This study lays the foundation for further research on the molecular mechanisms of anther growth and development in Chinese cabbage.
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影响因子：
4.4
作者：
Kang C;Liu Z
通讯作者：
Liu Z
影响因子：
4.3
作者：
Bohra, Abhishek;Gandham, Prasad;Singh, Narendra P.
通讯作者：
Singh, Narendra P.
影响因子：
28.2
作者：
Karreth FA;Pandolfi PP
通讯作者：
Pandolfi PP
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7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
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作者：
Huang, Li;Liu, Ying;Cao, Jiashu
通讯作者：
Cao, Jiashu