Overexpression of a Barley Aquaporin Gene, HvPIP2;5 Confers Salt and Osmotic Stress Tolerance in Yeast and Plants.
Overexpression of a Barley Aquaporin Gene, HvPIP2;5 Confers Salt and Osmotic Stress Tolerance in Yeast and Plants.
复制标题
DOI：
10.3389/fpls.2016.01566
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Panda SK
中科院分区：
文献类型：
--
作者：
Alavilli H;Awasthi JP;Rout GR;Sahoo L;Lee BH;Panda SK
我们从大麦中克隆了一个水通道蛋白基因HvPIP2；5，并研究了它在高盐和高渗透胁迫条件下在酵母和拟南芥等异源表达系统中的生理功能。在酵母中，HvPIP2；5的表达增强了在高盐和高渗透条件下非生物胁迫的耐受性。在高盐和高渗透胁迫下，高表达HvPIP2；5的拟南芥植株在萌发和根生长方面表现出比野生型(WT)更好的耐逆性。HvPIP2；5高表达植株能够在3周的干旱后存活和恢复，而对照植株在胁迫处理期间枯萎死亡。事实上，HvPIP2；5的过表达导致了在盐和渗透胁迫下比对照更高的叶绿素和水分的保留率。我们还观察到，在高表达HvPIP2；5的植物中，活性氧(ROS)和脂质过氧化的最终产物丙二醛(MDA)的积累比在WT中要低。这些结果表明，HvPIP2；5的过度表达至少在一定程度上通过减少盐和渗透胁迫引起的次生氧化应激而带来了胁迫耐受性。与这些耐胁迫表型一致的是，HvPIP2；5高表达拟南芥品系在盐和渗透胁迫下表现出比WT更高的ROS清除酶，如过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)和抗坏血酸过氧化物酶(APX)。此外，在盐胁迫和渗透胁迫下，Δ1-吡咯啉-5-羧酸合成酶1和2(P5CS1和P5CS2)基因在HvPIP2；5高表达植株中表达上调，这与渗透保护剂Pro水平的增加相一致。综上所述，这些结果表明，HvPIP2；5过表达通过增加ROS清除酶和渗透保护剂生物合成基因的活性和/或表达，增强了植物对高盐和高渗透胁迫的耐受性。
We characterized an aquaporin gene HvPIP2;5 from Hordeum vulgare and investigated its physiological roles in heterologous expression systems, yeast and Arabidopsis, under high salt and high osmotic stress conditions. In yeast, the expression of HvPIP2;5 enhanced abiotic stress tolerance under high salt and high osmotic conditions. Arabidopsis plants overexpressing HvPIP2;5 also showed better stress tolerance in germination and root growth under high salt and high osmotic stresses than the wild type (WT). HvPIP2;5 overexpressing plants were able to survive and recover after a 3-week drought period unlike the control plants which wilted and died during stress treatment. Indeed, overexpression of HvPIP2;5 caused higher retention of chlorophylls and water under salt and osmotic stresses than did control. We also observed lower accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), an end-product of lipid peroxidation in HvPIP2;5 overexpressing plants than in WT. These results suggest that HvPIP2;5 overexpression brought about stress tolerance, at least in part, by reducing the secondary oxidative stress caused by salt and osmotic stresses. Consistent with these stress tolerant phenotypes, HvPIP2;5 overexpressing Arabidopsis lines showed higher expression and activities of ROS scavenging enzymes such as catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), and ascorbate peroxidase (APX) under salt and osmotic stresses than did WT. In addition, the proline biosynthesis genes, Δ1-Pyrroline-5-Carboxylate Synthase 1 and 2 (P5CS1 and P5CS2) were up-regulated in HvPIP2;5 overexpressing plants under salt and osmotic stresses, which coincided with increased levels of the osmoprotectant proline. Together, these results suggested that HvPIP2;5 overexpression enhanced stress tolerance to high salt and high osmotic stresses by increasing activities and/or expression of ROS scavenging enzymes and osmoprotectant biosynthetic genes.
登录
查看更多内容
影响因子：
7.4
作者：
Chaumont, F;Barrieu, F;Jung, R
通讯作者：
Jung, R
影响因子：
4.9
作者：
Horie, Tomoaki;Kaneko, Toshiyuki;Katsuhara, Maki
通讯作者：
Katsuhara, Maki
影响因子：
44.1
作者：
Guo, Lei;Wang, Zi Yi;Gu, Hongya
通讯作者：
Gu, Hongya
影响因子：
3.7
作者：
Hove RM;Ziemann M;Bhave M
通讯作者：
Bhave M
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF